Please use this identifier to cite or link to this item: http://hdl.handle.net/10216/98164
Author(s): Faria, Ana
Pestana, D
Teixeira, D
Azevedo, J
Victor de Freitas
Nuno Mateus
Conceicao Calhau
Title: Flavonoid transport across RBE4 cells: A blood-brain barrier model
Issue Date: 2010
Abstract: There is a growing interest in dietary therapeutic strategies to combat oxidative stress-induced damage to the Central Nervous System (CNS), which is associated with a number of pathophysiological processes, including Alzheimer's and Parkinson's diseases and cerebrovascular diseases. Identifying the mechanisms associated with phenolic neuroprotection has been delayed by the lack of information concerning the ability of these compounds to enter the CNS. The aim of this study was to evaluate the transmembrane transport of flavonoids across RBE-4 cells (an immortalized cell line of rat cerebral capillary endothelial cells) and the effect of ethanol on this transport. The detection and quantification of all of the phenolic compounds in the studied samples (basolateral media) was performed using a HPLC-DAD (Diode Array Detector). All of the tested flavonoids (catechin, quercetin and cyanidin-3-glucoside) passed across the RBE-4 cells in a time-dependent manner. This transport was not influenced by the presence of 0.1% ethanol. In conclusion, the tested flavonoids were capable of crossing this blood-brain barrier model.
Subject: Biologia molecular, Espectroquímica, Citoquímica, Engenharia química
Molecular biology, Spectrochemistry, Cytochemistry, Chemical engineering
URI: http://hdl.handle.net/10216/98164
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FCUP - Artigo em Revista Científica Internacional
FMUP - Artigo em Revista Científica Internacional
FCNAUP - Artigo em Revista Científica Internacional

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