Iron-citrate can induce cell senescent phenotype in human fibroblasts in vitro

AbstractAfter a number of replications, human diploid fibroblasts (HDFs) in culture lose the ability to divide, become insensitive to further proliferation and enter a state of replicative senescence (RS). Subcytotoxic doses of several stressful agents such as hydrogen peroxide, tertbutylhydroperoxide or ethanol, are able to cause stress-induced premature senescence (SIPS) in HDFs in vitro. Such senescent cells display many features of RS as growth arrest, sen...

Risk communication and policymaking: examining participation context, digital media, and scientific evidence

Risk communication may shape how risks are defined and addressed by policy. This study examines stakeholders' risk communication practices within participatory approaches to policymaking through a multiple-case study design, covering both top-down and bottom-up pathways. The study pursues three interrelated objectives: to examine the forms of knowledge through which stakeholders communicate risk, including lived experience, situated accounts of perceived risk, and judgments of policy feasibil...

Microplastics and Antimicrobial Resistance Genes in Surface Waters Under European Union Regulatory Progress

Microplastics (MPs) and antimicrobial resistance genes (ARGs), emerging pollutants in surface waters, are viewed as a serious risk to freshwater ecosystems and public health. This review synthesizes current scientific knowledge, regulatory approaches, and monitoring methodologies on the presence and impact of these contaminants following a drivers-pressures-state-impact-response (DPSIR) framework. Major anthropogenic factors, such as pharmaceutical consumption and agricultural intensification...

Influence of apple pomace and asparaginase on acrylamide levels in cookies and behavior during simulated gastric digestion: risk assessment perspectives

Cookies are widely consumed baked products; however, their contribution to dietary acrylamide (ACR) intake raises increasing health concerns. This study investigated the effects of apple pomace addition and asparaginase treatment on ACR levels in cookies, the fate of ACR during simulated gastric digestion using the INFOGEST semi-dynamic model, and the associated health risks of ACR exposure from cookie consumption. Risk assessment was conducted for adults (18-65 years) and children (3-10 year...