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https://hdl.handle.net/10216/82098| Author(s): | Cortez, J Vorobieva, E Gralheira, D Osorio, I Soares, L Vale, N Pereira, E Gomes, P Franco, R |
| Title: | Bionanoconjugates of tyrosinase and peptide-derivatised gold nanoparticles for biosensing of phenolic compounds |
| Issue Date: | 2011 |
| Abstract: | Bionanoconjugates of the enzyme tyrosinase (TYR) and gold nanoparticles (AuNPs) functionalised with a peptide (CALNN) were produced in solution and characterised. The formation of stable TYR-AuNP:CALNN bionanoconjugates (BNCs) was supported by a decrease of the surface charge of the BNCs as determined by zeta-potential and an increase in hydrodynamic diameter as determined by Dynamic Light Scattering (DLS). UV/Vis studies of pH-induced aggregation revealed distinct protonation patterns for the BNCs when compared with AuNP:CALNN alone, further substantiating BNC formation. Activity studies of the BNCs for the reduction of di-phenols in solution indicated that TYR not only remains active after conjugation, but interestingly its activity in the BNCs is higher than for the free enzyme. In conclusion, AuNP:CALNN can provide a suitable platform for the immobilisation of TYR, leading to BNCs with increased enzyme activity and a wider pH working range, with promising uses in electrochemical biosensors for the detection of mono- and di-phenolic compounds. |
| Subject: | Engenharia dos materiais Materials engineering |
| Scientific areas: | Ciências da engenharia e tecnologias::Engenharia dos materiais Engineering and technology::Materials engineering |
| DOI: | 10.1007/s11051-010-0099-8 |
| URI: | https://repositorio-aberto.up.pt/handle/10216/82098 |
| Document Type: | Artigo em Revista Científica Internacional |
| Rights: | openAccess |
| License: | https://creativecommons.org/licenses/by-nc/4.0/ |
| Appears in Collections: | FCUP - Artigo em Revista Científica Internacional |
This item is licensed under a Creative Commons License
