Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/82098
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dc.creatorCortez, J
dc.creatorVorobieva, E
dc.creatorGralheira, D
dc.creatorOsorio, I
dc.creatorSoares, L
dc.creatorVale, N
dc.creatorPereira, E
dc.creatorGomes, P
dc.creatorFranco, R
dc.date.accessioned2019-02-01T10:59:13Z-
dc.date.available2019-02-01T10:59:13Z-
dc.date.issued2011
dc.identifier.issn1388-0764
dc.identifier.othersigarra:89163
dc.identifier.urihttps://repositorio-aberto.up.pt/handle/10216/82098-
dc.description.abstractBionanoconjugates of the enzyme tyrosinase (TYR) and gold nanoparticles (AuNPs) functionalised with a peptide (CALNN) were produced in solution and characterised. The formation of stable TYR-AuNP:CALNN bionanoconjugates (BNCs) was supported by a decrease of the surface charge of the BNCs as determined by zeta-potential and an increase in hydrodynamic diameter as determined by Dynamic Light Scattering (DLS). UV/Vis studies of pH-induced aggregation revealed distinct protonation patterns for the BNCs when compared with AuNP:CALNN alone, further substantiating BNC formation. Activity studies of the BNCs for the reduction of di-phenols in solution indicated that TYR not only remains active after conjugation, but interestingly its activity in the BNCs is higher than for the free enzyme. In conclusion, AuNP:CALNN can provide a suitable platform for the immobilisation of TYR, leading to BNCs with increased enzyme activity and a wider pH working range, with promising uses in electrochemical biosensors for the detection of mono- and di-phenolic compounds.
dc.language.isoeng
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectEngenharia dos materiais
dc.subjectMaterials engineering
dc.titleBionanoconjugates of tyrosinase and peptide-derivatised gold nanoparticles for biosensing of phenolic compounds
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoFaculdade de Ciências
dc.identifier.doi10.1007/s11051-010-0099-8
dc.identifier.authenticusP-002-THP
dc.subject.fosCiências da engenharia e tecnologias::Engenharia dos materiais
dc.subject.fosEngineering and technology::Materials engineering
Appears in Collections:FCUP - Artigo em Revista Científica Internacional

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