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https://hdl.handle.net/10216/82098
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DC Field | Value | Language |
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dc.creator | Cortez, J | |
dc.creator | Vorobieva, E | |
dc.creator | Gralheira, D | |
dc.creator | Osorio, I | |
dc.creator | Soares, L | |
dc.creator | Vale, N | |
dc.creator | Pereira, E | |
dc.creator | Gomes, P | |
dc.creator | Franco, R | |
dc.date.accessioned | 2019-02-01T10:59:13Z | - |
dc.date.available | 2019-02-01T10:59:13Z | - |
dc.date.issued | 2011 | |
dc.identifier.issn | 1388-0764 | |
dc.identifier.other | sigarra:89163 | |
dc.identifier.uri | https://repositorio-aberto.up.pt/handle/10216/82098 | - |
dc.description.abstract | Bionanoconjugates of the enzyme tyrosinase (TYR) and gold nanoparticles (AuNPs) functionalised with a peptide (CALNN) were produced in solution and characterised. The formation of stable TYR-AuNP:CALNN bionanoconjugates (BNCs) was supported by a decrease of the surface charge of the BNCs as determined by zeta-potential and an increase in hydrodynamic diameter as determined by Dynamic Light Scattering (DLS). UV/Vis studies of pH-induced aggregation revealed distinct protonation patterns for the BNCs when compared with AuNP:CALNN alone, further substantiating BNC formation. Activity studies of the BNCs for the reduction of di-phenols in solution indicated that TYR not only remains active after conjugation, but interestingly its activity in the BNCs is higher than for the free enzyme. In conclusion, AuNP:CALNN can provide a suitable platform for the immobilisation of TYR, leading to BNCs with increased enzyme activity and a wider pH working range, with promising uses in electrochemical biosensors for the detection of mono- and di-phenolic compounds. | |
dc.language.iso | eng | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | |
dc.subject | Engenharia dos materiais | |
dc.subject | Materials engineering | |
dc.title | Bionanoconjugates of tyrosinase and peptide-derivatised gold nanoparticles for biosensing of phenolic compounds | |
dc.type | Artigo em Revista Científica Internacional | |
dc.contributor.uporto | Faculdade de Ciências | |
dc.identifier.doi | 10.1007/s11051-010-0099-8 | |
dc.identifier.authenticus | P-002-THP | |
dc.subject.fos | Ciências da engenharia e tecnologias::Engenharia dos materiais | |
dc.subject.fos | Engineering and technology::Materials engineering | |
Appears in Collections: | FCUP - Artigo em Revista Científica Internacional |
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