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Author(s): Antunes, Patrícia
Campos, J
Mourão, J.
Pereira, J
Novais, C.
Peixe, L.
Title: Inflow water is a major source of trout farming contamination with Salmonella and multidrug resistant bacteria
Issue Date: 2018
Abstract: The impact of European aquaculture, namely trout farms, in the spread of antibiotic resistance and/or zoonotic pathogens has been scarcely addressed. Moreover, aquaculture contamination sources and bacterial dissemination routes have been barely explored. In this study, we assessed the contribution of Portuguese land-based intensive rainbow trout farms and retailed market trout to the spread of Salmonella and bacteria carrying clinically-relevant antibiotic resistance genes (ARGs) as well as inflow water and feed as possible sources of those contaminants. Cultural and molecular methods were used to analyse 53 fish farm samples (upstream/downstream water and sediments, tanks and trout) and 25 marketed trout. Plasmid-mediated quinolone resistance (PMQR) genes were found in 21% (n = 11/53) of samples (water/sediment/feed/trout), from all collection points (upstream/within/downstream tanks) and seasons, as well as in 12% (n = 3/25) of marketed trout (3 supermarkets). PMQR genes (qnrS1-S2-S3, qnrB7-B19, qnrD1, oqxAB) were detected in Enterobacteriaceae or Aeromonas hydrophila. An E. coli strain producing extended-spectrum-beta-lactamase SHV-12 was detected in all sampled points of a fish farm. Salmonella (4 serotypes, including S. Newport-ST118) was detected in 26% (n = 14/53) of the samples from both farms (water/sediment upstream/within tanks). The clinically-relevant plasmid-mediated colistin resistance mcr genes were not detected. However, colistin resistant S. Abony with new mutations in the chromosomal pmrA and pmrB genes was observed. Identical Salmonella and SHV-12-producing E. coli strains (by PFGE/MLST) in water upstream and within trout tanks points to inflow-water of trout farms as an important source of pathogenic bacteria and ARG contamination. These results highlight the need to define microbiological standards for water supplying fish farms in the EU and to establish surveillance and control strategies to limit bacterial transmission associated with this fastest growing food sector worldwide. © 2018 Elsevier B.V.
Subject: Ciências da Saúde, Ciências médicas e da saúde
Health sciences, Medical and Health sciences
Scientific areas: Ciências médicas e da saúde
Medical and Health sciences
Related Information: info:eu-repo/grantAgreement/Banco Santander Totta/Projectos Pluridisciplinares Iniciação à Investigação UP/IJUP_2012_2/The threat of multidrug resistance bacteria to public health: when aquacultures raise more than fish/Threat of multidrug
info:eu-repo/grantAgreement/CCRN - Comissão de Coordenação da Região Norte/P2020|Norte2020-Projetos Integrados ICDT/NORTE-01-0145-FEDER-000011/FOODnanoHEALTH /FOODnanoHEALTH
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Operacional Factores de Competitividade/PP_IJUP2011-81//Aquaculturas
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FCNAUP - Artigo em Revista Científica Internacional
FFUP - Artigo em Revista Científica Internacional

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