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https://hdl.handle.net/10216/100224
Full metadata record
DC Field | Value | Language |
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dc.creator | Faria, Ana | |
dc.creator | Pestana, D | |
dc.creator | Teixeira, D | |
dc.creator | Victor de Freitas | |
dc.creator | Nuno Mateus | |
dc.creator | Conceicao Calhau | |
dc.date.accessioned | 2019-02-02T09:38:49Z | - |
dc.date.available | 2019-02-02T09:38:49Z | - |
dc.date.issued | 2010 | |
dc.identifier.issn | 0951-418X | |
dc.identifier.other | sigarra:48609 | |
dc.identifier.uri | https://repositorio-aberto.up.pt/handle/10216/100224 | - |
dc.description.abstract | The purpose of this study was to investigate the anticancer properties of an anthocyanin-pyruvic acid adduct extract, which is being developed aiming to be further applied in the food industry. An anthocyanin extract from blueberry (extract I) and an anthocyanin-pyruvic acid adduct extract (extract II) were tested on two breast cancer cell lines (MDA-MB-231 and MCF7). Proliferation was assessed by SRB assay and H-3-thymidine incorporation. Caspase-3 activity was determined in the presence of both extracts. Their capacity as chemoattractants and their invasive potential were also assayed. In both cell lines, extracts I and II significantly reduced cell proliferation at 250 mu g/mL, after 24 h of cell incubation. Caspase-3 activity was not altered by the extracts (250 mu g/mL) in either cell line, with the exception of extract H in MCF-7, which increased its activity, probably explaining its effects on cell proliferation. Both extracts (250 mu g/mL) demonstrated significant antiinvasive potential in both cell lines. Furthermore, they did not demonstrate any capacity for chemotaxis. In conclusion, blueberry anthocyanins and the respective anthocyanin-pyruvic acid adducts demonstrated anticancer properties by inhibiting cancer cell proliferation and by acting as cell antiinvasive factors and chemoinhibitors. The anthocyanin-pyruvic acid adduct extract showed a more pronounced effect in MDA-MB-231, suggesting an effect independent of estrogen receptors. Copyright (C) 2010 John Wiley & Sons, Ltd. | |
dc.language.iso | eng | |
dc.rights | restrictedAccess | |
dc.subject | Química clínica, Citoquímica, Engenharia química | |
dc.subject | Clinical chemistry, Cytochemistry, Chemical engineering | |
dc.title | Blueberry Anthocyanins and Pyruvic Acid Adducts: Anticancer Properties in Breast Cancer Cell Lines | |
dc.type | Artigo em Revista Científica Internacional | |
dc.contributor.uporto | Faculdade de Ciências da Nutrição e Alimentação | |
dc.contributor.uporto | Faculdade de Medicina | |
dc.contributor.uporto | Faculdade de Ciências | |
dc.identifier.doi | 10.1002/ptr.3213 | |
dc.identifier.authenticus | P-003-0SH | |
dc.subject.fos | Ciências da engenharia e tecnologias::Engenharia química | |
dc.subject.fos | Engineering and technology::Chemical engineering | |
Appears in Collections: | FCNAUP - Artigo em Revista Científica Internacional FCUP - Artigo em Revista Científica Internacional FMUP - Artigo em Revista Científica Internacional |
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File | Description | Size | Format | |
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48609.pdf Restricted Access | 205.12 kB | Adobe PDF | Request a copy from the Author(s) |
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