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Author(s): Pancrazio, Gastón
Vieira, Elsa
Melo, Armindo
Ferreira, Isabel
Pinho, Olívia
Title: Proteolysis of sarcoplasmic pork meat proteins evaluated by high-performance liquid chromatography
Issue Date: 2013
Abstract: A reverse-phase high performance liquid chromatography method that enables simultaneous analysis of peptides and proteins was applied for routine monitorization of proteolytic changes that occur in sarcoplasmic pork meat proteins due to the contribution of endogenous and exogenous enzymes from yeast origin. The chromatograms obtained for sarcoplasmic protein extracts, with and without addition of yeast enzyme extracts were analyzed at 0 min incubation and after hydrolysates at 37ºC during 24 h. Qualitative and quantitative differences were observed in the chromatograms. Hydrolysis of sarcoplasmic extracts by endogenous enzymes lead to 33% of protein degradation and an increase of 124% in polypeptides of big dimensions, no significant formation of low molecular peptides and medium size peptides was observed. Yeast enzymatic extracts promoted increased hydrolysis of sarcoplasmic extracts (up to 57% of protein degradation) and an increase of 245% in polypeptides of big dimensions, 170% in low molecular peptides and 244% in medium size peptides.
Subject: Ciências da Saúde, Ciências da saúde
Health sciences, Health sciences
Scientific areas: Ciências médicas e da saúde::Ciências da saúde
Medical and Health sciences::Health sciences
Source: Livro de Actas do 59th International Congress of Meat Science and Technology
Document Type: Artigo em Livro de Atas de Conferência Internacional
Rights: restrictedAccess
Appears in Collections:FCNAUP - Artigo em Livro de Atas de Conferência Internacional

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