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Author(s): Claudia Marques
Diana Teixeira
Ana Cunha
Manuela Meireles
Diogo Pestana
Elisa Keating
Conceicao Calhau
Rosario Monteiro
Ana Faria
Title: Methotrexate enhances 3T3-L1 adipocytes hypertrophy
Issue Date: 2013
Abstract: Methotrexate (MTX) is broadly used in the treatment of chronic inflammatory diseases such as rheumatoid arthritis (RA). The prevalence of metabolic syndrome (MeS) in patients with this condition is relatively high. Given the importance of adipose tissue in the development of obesity metabolic complications, this study aimed to investigate the effect of methotrexate on preadipocyte proliferation, adipogenesis, and glucose uptake by adipocytes. 3T3-L1 preadipocytes proliferation was evaluated by sulforhodamine B staining and H-3-thymidine incorporation, after 24 or 48 h of treatment with MTX (0.1 and 10 mu M). Preadipocytes were induced to differentiate with an appropriate adipogenic cocktail in the presence or absence of MTX. Adipogenesis was determined by measuring lipid accumulation after staining with oil red O. H-3-Deoxyglucose (H-3-DG) uptake was determined by liquid scintillation counting. MTX treatment reduced culture protein content in a concentration-dependent manner and H-3-thymidine incorporation (P < 0.05). MTX (0.1 mu M) treatment increased lipid accumulation and basal H-3-DG uptake by adipocytes (P < 0.05). In 0.1 mu M MTX-treated adipocytes, insulin stimulation did not result in an increase of H-3-DG uptake, contrarily to what was observed in control cells. These results demonstrate that methotrexate interferes with adipocyte proliferation and promotes the hypertrophic growth of adipocytes. These molecular effects may have implications on metabolic profile of RA patients treated with MTX.
Subject: Ciências da Saúde, Medicina básica
Health sciences, Basic medicine
Scientific areas: Ciências médicas e da saúde::Medicina básica
Medical and Health sciences::Basic medicine
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FCUP - Artigo em Revista Científica Internacional
FMUP - Artigo em Revista Científica Internacional

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