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https://hdl.handle.net/10216/100164
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DC Field | Value | Language |
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dc.creator | Ana Faria | |
dc.creator | Manuela Meireles | |
dc.creator | Iva Fernandes | |
dc.creator | Celestino Santos Buelga | |
dc.creator | Susana Gonzalez Manzano | |
dc.creator | Montserrat Duenas | |
dc.creator | Victor de Freitas | |
dc.creator | Nuno Mateus | |
dc.creator | Conceicao Calhau | |
dc.date.accessioned | 2022-09-13T23:09:08Z | - |
dc.date.available | 2022-09-13T23:09:08Z | - |
dc.date.issued | 2014 | |
dc.identifier.issn | 0308-8146 | |
dc.identifier.other | sigarra:47923 | |
dc.identifier.uri | https://hdl.handle.net/10216/100164 | - |
dc.description.abstract | This study aimed to evaluate the transmembrane transport of different flavonoids (flavan-3-ols, anthocyanins and flavonols) and some of their metabolites (methylated and conjugated with glucuronic acid) across hCMEC/D3 cells (a blood-brain barrier (BBB) model). Further metabolism of the tested compounds was assayed and their transport modulated in an attempt to elucidate the mechanisms behind this process. The transport across hCMEC/D3 cells was monitored in basolateral media at 1, 3 and 18 h by HPLC-DAD/MS. All the flavonoids and their metabolites were transported across hCMEC/D3 cells in a time-dependent manner. In general, the metabolites showed higher transport efficiency than the native flavonoid. No further biotransformation of the metabolites was found as consequence of cellular metabolism. Anthocyanins and their metabolites crossed this BBB cell model in a lipophilicity-dependent way. Quercetin transport was influenced by phosphatase modulators, suggesting a phosphorylation/dephosphorylation regulation mechanism. Overall, this work suggests that flavonoids are capable of crossing the BBB and reaching the central nervous system. | |
dc.language.iso | eng | |
dc.rights | restrictedAccess | |
dc.subject | Outras ciências agrárias | |
dc.subject | Other Agrarian Sciences | |
dc.title | Flavonoid metabolites transport across a human BBB model | |
dc.type | Artigo em Revista Científica Internacional | |
dc.contributor.uporto | Faculdade de Ciências | |
dc.contributor.uporto | Faculdade de Medicina | |
dc.identifier.doi | 10.1016/j.foodchem.2013.10.095 | |
dc.identifier.authenticus | P-008-GSJ | |
dc.subject.fos | Ciências agrárias::Outras ciências agrárias | |
dc.subject.fos | Agrarian Sciences::Other Agrarian Sciences | |
Appears in Collections: | FCUP - Artigo em Revista Científica Internacional FMUP - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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47923.pdf Restricted Access | 510.94 kB | Adobe PDF | Request a copy from the Author(s) |
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