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dc.creatorAna Faria
dc.creatorManuela Meireles
dc.creatorIva Fernandes
dc.creatorCelestino Santos Buelga
dc.creatorSusana Gonzalez Manzano
dc.creatorMontserrat Duenas
dc.creatorVictor de Freitas
dc.creatorNuno Mateus
dc.creatorConceicao Calhau
dc.description.abstractThis study aimed to evaluate the transmembrane transport of different flavonoids (flavan-3-ols, anthocyanins and flavonols) and some of their metabolites (methylated and conjugated with glucuronic acid) across hCMEC/D3 cells (a blood-brain barrier (BBB) model). Further metabolism of the tested compounds was assayed and their transport modulated in an attempt to elucidate the mechanisms behind this process. The transport across hCMEC/D3 cells was monitored in basolateral media at 1, 3 and 18 h by HPLC-DAD/MS. All the flavonoids and their metabolites were transported across hCMEC/D3 cells in a time-dependent manner. In general, the metabolites showed higher transport efficiency than the native flavonoid. No further biotransformation of the metabolites was found as consequence of cellular metabolism. Anthocyanins and their metabolites crossed this BBB cell model in a lipophilicity-dependent way. Quercetin transport was influenced by phosphatase modulators, suggesting a phosphorylation/dephosphorylation regulation mechanism. Overall, this work suggests that flavonoids are capable of crossing the BBB and reaching the central nervous system.
dc.subjectOutras ciências agrárias
dc.subjectOther Agrarian Sciences
dc.titleFlavonoid metabolites transport across a human BBB model
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoFaculdade de Medicina
dc.contributor.uportoFaculdade de Ciências
dc.subject.fosCiências agrárias::Outras ciências agrárias
dc.subject.fosAgrarian Sciences::Other Agrarian Sciences
Appears in Collections:FCUP - Artigo em Revista Científica Internacional
FMUP - Artigo em Revista Científica Internacional

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