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Author(s): Domingues, A.
Nobre, C.
Rodrigues, L.R.
Peres, A.M.
Torres, Duarte
Rocha, I.
Lima, N.
Teixeira, José
Title: New improved method for fructooligosaccharides production by Aureobasidium pullulans
Issue Date: 2012
Abstract: Fructooligosaccharides are prebiotics with numerous health benefits within which the improvement of gut microbiota balance can be highlighted, playing a key role in individual health. In this study, an integrated one-stage method for FOS production via sucrose fermentation by Aureobasidium pullulans was developed and optimized using experimental design tools. Optimization of temperature and agitation speed for maximizing the FOS production was performed using response surface methodology. Temperature was found to be the most significant parameter. The optimum fermentation conditions were found to be 32 degrees C and 385 rpm. Under these conditions, the model predicted a total FOS production yield of 64.7 g(FOS)/g(sucrose). The model was validated at optimal conditions in order to check its adequacy and accuracy and an experimental yield of 64.1 (+/- 0.0)g(FOS)/g(sucrose) was obtained. A significant improvement of the total FOS production yields by A. pullulans using a one-stage process was obtained. (C) 2012 Elsevier Ltd. All rights reserved.
Subject: Biotecnologia alimentar, Biotecnologia agrária e alimentar
Food biotechnology, Agricultural biotechnology
Scientific areas: Ciências agrárias::Biotecnologia agrária e alimentar
Agrarian Sciences::Agricultural biotechnology
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FCNAUP - Artigo em Revista Científica Internacional

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