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Author(s): Fatima Paiva Martins
Joao Fernandes
Vera Santos
Lisete Silva
Fernanda Borges
Susana Rocha
Luis Belo
Alice Santos Silva
Title: Powerful Protective Role of 3,4-Dihydroxyphenylethanol-Elenolic Acid Dialdehyde against Erythrocyte Oxidative-Induced Hemolysis
Issue Date: 2010
Abstract: The present work studied and compared the capacity of four important olive oil polyphenolic compounds, oleuropein, hydroxytyrosol, and the oleuropein aglycones 3,4-dihydroxyphenylethanol-elenolic acid (3,4-DHPEA-EA) and 3,4-dihydroxyphenylethanol-elenolic acid dialdehyde (3,4-DHPEA-EDA), to protect red blood cells (RBCs) from oxidative hemolysis induced by the physiological initiator H(2)O(2). The amount of hemolysis was evaluated spectrophotometrically. The compounds were also tested in the presence and absence of the naturally occurring antioxidant ascorbic acid. All compounds were revealed to significantly protect RBCs from oxidative hemolysis induced by H(2)O(2) at 40 and 80 mu M, with the order of activity being 3,4-DHPEA-EDA > 3,4-DHPEA-EA > hydroxytyrosol = oleuropein. At 20, 10, and 5 mu M, only 3,4-DHPEA-EDA showed a significant protection against the oxidative injury. In the presence of ascorbic acid at physiological concentration, the addition of individual compounds at 40 mu M increased the stability of erythrocytes. The addition of phenolic compounds at 20 and 10 mu M did not produce further protection when compared with the protection given by ascorbic acid alone, except for 3,4-DHPEA-EDA. This compound was shown to produce further protection even at 5 mu M. In summary, 3,4-DHPEA-EDA plays an important protective role against reactive oxygen species-induced oxidative injury in RBCs, and this effect is more potent than the one evidenced by hydroxytyrosol or oleuropein.
Subject: Outras ciências agrárias
Other Agrarian Sciences
Scientific areas: Ciências agrárias::Outras ciências agrárias
Agrarian Sciences::Other Agrarian Sciences
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FCUP - Artigo em Revista Científica Internacional
FFUP - Artigo em Revista Científica Internacional

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