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Author(s): João M. M. Leitão
Joaquim C. G. Esteves da Silva
Title: Firefly luciferase inhibition
Issue Date: 2010
Abstract: Firefly luciferase (Luc) is the most studied of the luciferase enzymes and the mechanism and kinetics ofthe reactions catalyzed by this enzyme have been relatively well characterized. Luc catalyzes the bioluminescentreaction involving firefly luciferin (D-LH2), adenosine triphosphate (ATP), magnesium ion andmolecular oxygen with the formation of an electronically excited species (oxyluciferin), inorganic pyrophosphate(PPi), carbon dioxide and adenosine monophosphate (AMP). Luc also catalyzes other nonluminescentreactions, which can interfere with the light production mechanism. Following electronicrelaxation, the excited oxyluciferin emits radiation in the visible region of the electromagnetic spectrum(550-570 nm). Among the various possible compounds, several classes of inhibitory substances interferewith the activity of this enzyme: here, we consider substrate-related compounds, intermediates or productsof the Luc catalyzed reactions, in addition to anesthetics and, fatty acids. This review summarizes themain inhibitors of Luc and the corresponding inhibition kinetic parameters.
Subject: Enzimologia, Espectroquímica, Engenharia química
Enzimology, Spectrochemistry, Chemical engineering
Scientific areas: Ciências da engenharia e tecnologias::Engenharia química
Engineering and technology::Chemical engineering
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FCUP - Artigo em Revista Científica Internacional

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