Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/93867
Author(s): Soares, J
Coimbra, AM
Reis Henriques, MA
Monteiro, NM
Vieira, MN
Oliveira, JMA
Guedes Dias, P
Fontainhas Fernandes, A
Silva S Parra
Carvalho, AP
Filipe F C Castro
Santos, MM
Title: Disruption of zebrafish (Danio rerio) embryonic development after full life-cycle parental exposure to low levels of ethinylestradiol
Issue Date: 2009
Abstract: Exposure of fish to the synthetic estrogen ethinylestradiol (EE(2)) has been shown to induce a large set of deleterious effects. In addition to the negative impact of EE2 in reproductive endpoints, concern has recently increased on the potential effects of EE2 in fish embryonic development. Therefore, the present study aimed at examining the effects of EE2 on the full embryonic development of zebrafish in order to identify the actual phases where EE2 disrupts this process. Hence, zebrafish were exposed to environmentally relevant low levels of EE2, 0.5, 1 and 2 ng/L (actual concentrations of 0.19, 0.24 and 1 ng/L, respectively) from egg up to eight months of age (F(1)), and the survival as well as the occurrence of abnormalities in their offsprings (F(2)), per stage of embryonic development, was investigated. A thorough evaluation of reproductive endpoints and transcription of vtg1 gene in the parental generation (F(1)) at adulthood, was performed. No significant differences could be observed for the two lowest EE2 treatments, in comparison with controls, whereas vtg1 transcripts were significantly elevated (40-fold) in the 2 ng/L EE2 treatment. In contrast to the findings in the F(1) generation,a significant concentration-dependent increase in egg mortality between 8 and 24 hours post-fertilization (hpf) was observed for all EE2 treatments, when compared with controls. The screening of egg and embryo development showed a significant increase in the percentage of abnormalities at 8 hpf for the highest EE2 concentration, a fact that might explain the increased embryo mortality at the 24 hpf time-point observation. Taken together, these findings indicate that the two lowest tested EE2 concentations impact late gastrulation and/or early organogenesis, whereas exposure to 2 ng/L EE2 also disrupts development in the blastula phase. After early organogenesis has been completed (24 hpf), no further mortality was observed. These results show that increased embryo mortality occurs at EE2 levels below those inducing reproductive impairment and vtg1 gene induction in the male parental generation, thus suggesting that EE2 may impact some fish populations at levels below those inducing an increase in vtg1 transcripts. Hence, these findings have important implications for environmental risk assessment, strongly supporting the inclusion of embryonic development studies in the screening of endocrine disruption in wild fish populations.
Description: Exposure of fish to the synthetic estrogen ethinylestradiol (EE2) has been shown to induce a large set of deleterious effects. In addition to the negative impact of EE2 in reproductive endpoints, concern has recently increased on the potential effects of EE2 in fish embryonic development. Therefore, the present study aimed at examining the effects of EE2 on the full embryonic development of zebrafish in order to identify the actual phases where EE2 disrupts this process. Hence, zebrafish were exposed to environmentally relevant low levels of EE2, 0.5, 1 and 2 ng/L (actual concentrations of 0.19, 0.24 and 1 ng/L, respectively) from egg up to eight months of age (F1), and the survival as well as the occurrence of abnormalities in their offsprings (F2), per stage of embryonic development, was investigated. A thorough evaluation of reproductive endpoints and transcription of vtg1 gene in the parental generation (F1) at adulthood, was performed. No significant differences could be observed for the two lowest EE2 treatments, in comparison with controls, whereas vtg1 transcripts were significantly elevated (40-fold) in the 2 ng/L EE2 treatment. In contrast to the findings in the F1 generation,a significant concentration-dependent increase in egg mortality between 8 and 24 hours post-fertilization (hpf) was observed for all EE2 treatments, when compared with controls. The screening of egg and embryo development showed a significant increase in the percentage of abnormalities at 8 hpf for the highest EE2 concentration, a fact that might explain the increased embryo mortality at the 24 hpf time-point observation. Taken together, these findings indicate that the two lowest tested EE2 concentations impact late gastrulation and/or early organogenesis, whereas exposure to 2 ng/L EE2 also disrupts development in the blastula phase. After early organogenesis has been completed (24 hpf), no further mortality was observed. These results show that increased embryo mortality occurs at EE2 levels below those inducing reproductive impairment and vtg1 gene induction in the male parental generation, thus suggesting that EE2 may impact some fish populations at levels below those inducing an increase in vtg1 transcripts. Hence, these findings have important implications for environmental risk assessment, strongly supporting the inclusion of embryonic development studies in the screening of endocrine disruption in wild fish populations.<br><br>Keywords: Zebrafish; Ethinylestradiol; Embryonic development; Endocrine disruptor; Estrogenic chemical; Fish; Vitellogenin; Environmental risk assessment<br><a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T4G-4WXSJWB-1&_user=2460038&_coverDate=12%2F13%2F2009&_rdoc=11&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info(%23toc%234974%232009%23999049995%231571241%23FLA%23display%23Volume)&_cdi=4974&_sort=d&_docanchor=&_ct=14&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=7646e62715aace619679f6cc302f36db&searchtype=a"> Texto integral</a><br><bR>
Subject: Ciências farmacológicas, Medicina básica
Scientific areas: Ciências médicas e da saúde::Medicina básica
URI: http://hdl.handle.net/10216/93867
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FCUP - Artigo em Revista Científica Internacional
FFUP - Artigo em Revista Científica Internacional
ICBAS - Artigo em Revista Científica Internacional

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