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Author(s): Antunes, Patrícia
Coque, Teresa M.
Peixe, Luísa
Title: Emergence of an IncIg plasmid encoding CMY-2 b-lactamaseassociated with the international ST19 OXA-30-producing b-lactamaseSalmonella Typhimurium multidrug-resistant clone
Issue Date: 2010
Abstract: Objectives: To analyse the genetic environment of the blaCMY-2 gene in a multidrug-resistant isolate belongingto the OXA-30-producing Salmonella enterica serotype Typhimurium clone widespread in European countries.Methods: Preliminary characterization of CMY-2 was performed after determining the phenotype againstb-lactams, the pI (isoelectric focusing) and the presence of bla genes (PCR). The genetic context of theblaCMY-2 gene was identified by PCR mapping and further sequencing. Plasmid analysis included determinationof transferability, size and content (S1-PFGE/hybridization), and characterization of the incompatibility group(PCR-based replicon typing, hybridization and sequencing).Results: The DISEcp1-blaCMY-2-blc-sugE cassette identified is located in the pndC-trbA region of an IncIgconjugative plasmid designated pSTHV23035. This plasmid differs from previously described European IncIplasmids coding for CMY in the IncI replicon type and in the integration site of the element carrying blaCMY-2.Conclusions: The emergence of blaCMY-2 located in a new IncIg plasmid in an isolate belonging to the internationalwidespread multidrug-resistant sequence type (ST) 19 OXA-30-producing Salmonella Typhimuriumclone is reported for the first time. Acquisition of a new blaCMY-2-IncIg plasmid by Salmonella multidrugresistantstrains is of concern since these plasmids diminish the activity of therapeutically important broadspectrumb-lactams and contribute to the persistence and pathogenicity of widespread clones.
Subject: Ciências da Saúde, Outras ciências médicas
Health sciences, Other medical sciences
Scientific areas: Ciências médicas e da saúde::Outras ciências médicas
Medical and Health sciences::Other medical sciences
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FCNAUP - Artigo em Revista Científica Internacional

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