Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/92971
Author(s): Carina Pinho
Zita E Martins
Catarina Petisca
Agata M Figurska
Olivia Pinho
Isabel M P L V O Ferreira
Title: Size exclusion and reversed-phase high-performance liquid chromatography/UV for routine control of thermal processing of cows' and donkey milk major proteins
Issue Date: 2012
Abstract: Cows' and donkey milks (raw and thermally processed) and respective whey were analysed for quantification of major proteins. Two different chromatographic approaches, size exclusion (SE-HPLC) and reversed-phase high performance liquid chromatography (RP-HPLC) both coupled to UV detection were used. Usefulness of these methods for routine control of the effect of thermal processing was evaluated. The external standard method was used to calibrate the SE-HPLC and RP-HPLC systems. Concerning quantification of beta-lactoglobulin (beta-lg), alpha-lactalbumin (alpha-la), lysozyme (lys), and total casein (cn), no significant differences between results obtained by SE-HPLC and by RP-HPLC (t-test, P>0.05) were observed for raw milks and whey. Heating of cows' milk promoted aggregation of denatured proteins as observed by SE-HPLC, whereas alpha-la and beta-lg from donkey milk were stable to thermal processing at 100 degrees C (5 min). Lys was quantified in donkey raw milk and whey however, in thermally processed donkey milk lys was denatured and could not be quantified by HPLC.
Subject: Ciências da Saúde, Ciência animal e dos lacticínios
Health sciences, Animal and Dairy science
Scientific areas: Ciências agrárias::Ciência animal e dos lacticínios
Agrarian Sciences::Animal and Dairy science
URI: https://repositorio-aberto.up.pt/handle/10216/92971
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FCNAUP - Artigo em Revista Científica Internacional
FFUP - Artigo em Revista Científica Internacional

Files in This Item:
File Description SizeFormat 
46890.pdf
  Restricted Access
298.29 kBAdobe PDF    Request a copy from the Author(s)


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.