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https://hdl.handle.net/10216/86608
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DC Field | Value | Language |
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dc.creator | J Mourão | |
dc.creator | Marçal, Sara | |
dc.creator | Ramos, Paula | |
dc.creator | Campos, Joana | |
dc.creator | Machado, Jorge | |
dc.creator | Luisa Peixe | |
dc.creator | Novais, Carla | |
dc.creator | Antunes, Patrícia | |
dc.date.accessioned | 2022-09-08T11:26:09Z | - |
dc.date.available | 2022-09-08T11:26:09Z | - |
dc.date.issued | 2016 | |
dc.identifier.issn | 0305-7453 | |
dc.identifier.other | sigarra:167467 | |
dc.identifier.uri | https://hdl.handle.net/10216/86608 | - |
dc.description.abstract | Objectives: Factors driving the expansion of particular MDR Salmonella serotypes/clones are not completely understood. We assessed if emergent MDR Salmonella serotypes/clones were more enriched in metal tolerance genes (e.g. to Cu/Ag) than other less frequent ones, as an additional feature to survive in environments contaminated with metals. Methods: Metal (Cu pco/Ag,Cu sil/Hg mer/As ars/Te ter) tolerance genes screening (PCR/sequencing), MICs of CuSO4/AgNO3 (aerobiosis/anaerobiosis), genetic element characterization (S1/I-CeuI PFGE) and conjugation assays were performed in a well-characterized Salmonella collection (n275 isolates; 200014; 49 serotypes/clones). Results: The sil+pco genes were detected in 37% of isolates from diverse serotypes, mainly in emergent Rissen/ST469 and Typhimurium/ST34 European clone (100%), which are mostly associated with pig settings where Cu is highly used. These genes were frequently co-located with merA+terFand/or antibiotic resistance genes in plasmids (100270 kb; IncHI2/IncHI1/IncN/IncFIIA; mostly transferable by conjugation) or in the chromosome. Most sil+pco+ isolates (77%) were MDR contrasting with sil+pco2 ones (48%). The sil+pco+ isolates presented significantly higher MICCuSO4 under anaerobiosis (MIC50/MIC9028/32 mM) and MICAgNO3 after previous Ag contact (MIC50/MIC90.3 mM) than sil2 ones (MIC50/MIC902/8 mM to CuSO4; MIC50/MIC900.125/0.16 mM to AgNO3). Use of these modified methodological approaches allowed the establishment of CuSO4/AgNO3 tolerance cut-offs to differentiate sil+ and sil2 isolates, here firstly proposed. Conclusions: This study demonstrates that acquisition of Cu/Ag tolerance genes (sil/pco genes) might contribute to the emergence of particular clinically relevant MDR Salmonella serotypes/clones by facilitating their survival in diverse metal-contaminated settings, particularly in pig production. Assessment of control measures for the use and/or accumulation of metals in diverse environments are needed to prevent a wider expansion of such strains or the emergence of new ones. | |
dc.language.iso | eng | |
dc.rights | restrictedAccess | |
dc.subject | Ciências da Saúde, Ciências médicas e da saúde | |
dc.subject | Health sciences, Medical and Health sciences | |
dc.title | Tolerance to multiple metal stressors in emerging non-typhoidal MDR Salmonella serotypes: a relevant role for copper in anaerobic conditions | |
dc.type | Artigo em Revista Científica Internacional | |
dc.contributor.uporto | Faculdade de Farmácia | |
dc.contributor.uporto | Faculdade de Ciências da Nutrição e Alimentação | |
dc.identifier.doi | 10.1093/jac/dkw120 | |
dc.identifier.authenticus | P-00M-0CS | |
dc.subject.fos | Ciências médicas e da saúde | |
dc.subject.fos | Medical and Health sciences | |
Appears in Collections: | FCNAUP - Artigo em Revista Científica Internacional FFUP - Artigo em Revista Científica Internacional |
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File | Description | Size | Format | |
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167467.pdf Restricted Access | 301.87 kB | Adobe PDF | Request a copy from the Author(s) |
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