Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/82116
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dc.creatorGomes, P
dc.creatorGiralt, E
dc.creatorAndreu, D
dc.date.accessioned2019-02-03T11:02:12Z-
dc.date.available2019-02-03T11:02:12Z-
dc.date.issued2001
dc.identifier.issn0264-410X
dc.identifier.othersigarra:89198
dc.identifier.urihttps://repositorio-aberto.up.pt/handle/10216/82116-
dc.description.abstractFoot-and-mouth disease virus (FMDV) isolate C-1-Barcelona (or C-S30) includes four replacements within its immunodominant site (GH loop, residues 136-150 of capsid protein VP1, YTTSTRGDLAHVTAT), relative to reference strain C-S8cl (YTASAR-GDLAHLTTT). Although one of the mutations in C-S30 ((147)Leu --> Val) is known to be detrimental for antibody recognition, reactivity of this isolate with the neutralizing monoclonal antibody (mAb) 4C4, raised against FMDV C-1-Brescia (GH loop: YTASTRGDLAHLTAT), was indistinguishable from those of strains C-S8cl or C-1-Brescia. A structural interpretation for these somewhat striking findings is available, based on the observation that 15-residue peptides reproducing the C-S30 and C-S8cl GH loops adopt very similar, quasi-circular, conformations in crystal complexes with 4C4. Nevertheless, surface plasmon resonance (SPR) kinetic analyses of the interactions between these peptides and three anti-GH loop mAbs have now revealed that the linear C-S30 peptides were less antigenic in solution than their C-S8cl and C-1-Brescia counterparts. We have, therefore, tried to modulate peptide antigenicity in solution by cyclization. Functional SPR and structural two dimensional proton nuclear magnetic resonance (2D-H-1 NMR) studies of both linear and cyclic peptide antigens are discussed here. Conformation seems to have an important role in peptide antigenicity, even when continuous (i.e. linear) antigenic sites are involved.
dc.language.isoeng
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectMedicina básica
dc.subjectBasic medicine
dc.titleAntigenicity modulation upon peptide cyclization: application to the GH loop of foot-and-mouth disease virus strain C-1-Barcelona
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoFaculdade de Ciências
dc.identifier.doi10.1016/s0264-410x(01)00047-0
dc.identifier.authenticusP-007-9ZQ
dc.subject.fosCiências médicas e da saúde::Medicina básica
dc.subject.fosMedical and Health sciences::Basic medicine
Appears in Collections:FCUP - Artigo em Revista Científica Internacional

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