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https://hdl.handle.net/10216/128679
Author(s): | Marina Barroso Pereira Pinheiro |
Title: | Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model |
Issue Date: | 2020-05-11 |
Abstract: | Aim: Design nanostructured lipid carriers (NLC) to facilitate drug delivery to tuberculosis-infected areas, exploiting macrophage mannose receptors and assess their uptake in a 3D human lung model. Materials & methods: NLCs and mannosylated-NLCs were synthetized and characterized. Their uptake and biocompatibility were tested in a 3D human lung model. Results: The formulations have appropriate size (170-202 nm) and morphology for lung deposition. Cell membrane integrity was maintained and no significant pro-inflammatory cytokine (IL-1β, IL-8 and TNF-α) secretion or morphological changes were observed 24 h post nanoparticles exposure. NLCs and mannosylated NLCs were distributed in the apical side of the lung tissue, both in macrophages and in epithelial cells. Conclusion: NLCs are biocompatible carriers and can be used for pulmonary drug delivery. |
Description: | Aim: Design nanostructured lipid carriers (NLC) to facilitate drug delivery to tuberculosis-infected areas, exploiting macrophage mannose receptors and assess their uptake in a 3D human lung model. Materials & methods: NLCs and mannosylated-NLCs were synthetized and characterized. Their uptake and biocompatibility were tested in a 3D human lung model. Results: The formulations have appropriate size (170-202 nm) and morphology for lung deposition. Cell membrane integrity was maintained and no significant pro-inflammatory cytokine (IL-1β, IL-8 and TNF-α) secretion or morphological changes were observed 24 h post nanoparticles exposure. NLCs and mannosylated NLCs were distributed in the apical side of the lung tissue, both in macrophages and in epithelial cells. Conclusion: NLCs are biocompatible carriers and can be used for pulmonary drug delivery. |
Subject: | Biotecnologia médica Medical biotechnology |
Scientific areas: | Ciências médicas e da saúde::Biotecnologia médica Medical and Health sciences::Medical biotechnology |
TID identifier: | 202616444 |
URI: | https://hdl.handle.net/10216/128679 |
Document Type: | Dissertação |
Rights: | openAccess |
License: | https://creativecommons.org/licenses/by-sa/4.0/ |
Appears in Collections: | FMUP - Dissertação |
Files in This Item:
File | Description | Size | Format | |
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414414.pdf | Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model | 10.82 MB | Adobe PDF | View/Open |
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