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Author(s): Sílvia C. Pinho
Olga C. Nunes
Alexandre Lobo da Cunha
Manuel F. Almeida
Title: Inactivation of Geobacillus stearothermophilus spores by alkaline hydrolysis applied to medical waste treatment
Issue Date: 2015
Abstract: Although alkaline hydrolysis treatment emerges as an alternative disinfection/sterilization method for medical waste, information on its effects on the inactivation of biological indicators is scarce. The effects of alkaline treatment on the resistance of Geobacillus stearothermophilus spores were investigated and the influence of temperature (80 degrees C, 100 degrees C and 110 degrees C) and NaOH concentration was evaluated. In addition, spore inactivation in the presence of animal tissues and discarded medical components, used as surrogate of medical waste, was also assessed. The effectiveness of the alkaline treatment was carried out by determination of survival curves and D-values. No significant differences were seen in D-values obtained at 80 degrees C and 100 degrees C for NaOH concentrations of 0.5 M and 0.75 M. The D-values obtained at 110 degrees C (2.3-0.5 min) were approximately 3 times lower than those at 100 degrees C (8.8-1.6 min). Independent of the presence of animal tissues and discarded medical components, 6 log10 reduction times varied between 66 and 5 min at 100 degrees C-0.1 M NaOH and 110 degrees C-1 M NaOH, respectively. The alkaline treatment may be used in future as a disinfection or sterilization alternative method for contaminated waste.
Subject: Biologia ambiental, Tecnologia ambiental, Engenharia do ambiente, Ciências biológicas
Environmental biology, Environmental technology, Environmental engineering, Biological sciences
Scientific areas: Ciências da engenharia e tecnologias::Engenharia do ambiente
Engineering and technology::Environmental engineering
Ciências exactas e naturais::Ciências biológicas
Natural sciences::Biological sciences
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/SAU-SAP/114855/2009/Hidrólise alcalina de resíduos hospitalares infecciosos/PTDC/SAU-SAP/114855/2009
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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