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Author(s): Neri, David
Balcão, Victor
Costa, Rafael
Ferreira, Eugénio
Torres, Duarte
Rodrigues, Lígia
Carvalho Jr., Luiz
Teixeira, José
Title: β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: a comparative experimental and modelling study of the galactooligosaccharides production.
Issue Date: 2008
Abstract: β-Galactosidase from Aspergillus oryzae is an enzyme with a wide industrial application, mostly in the hydrolysis of lactose and, more recently, in the synthesis of oligosaccharides. Several advantages are associated with the application of immobilized enzymes on magnetic supports. In this work, β-galactosidase was covalently immobilised onto a Polysiloxane-Polyvinyl Alcohol Magnetic Composite (mPOS-PVA), Magnetic Polysiloxane with Polyaniline (mPOS-PANI), Magnetized Dacron (DACRON) and Magnetite with Polyaniline (MAG-PANI) using glutaraldehyde as activating agent being the synthesis of GOS evaluated and compared at different temperatures (30, 40, 50 and 60 oC) and various initial lactose concentration (50, 100, 200, 300, 400 and 500 g/L). The kinetic parameters obtained by fitting the experimental data were compared in order to determine the effect of the immobilization process with different supports on the synthesis of oligosaccharides. These results clearly demonstrate that all supports may be used for β-galactosidase immobilization as, besides improving the enzyme hydrolytic and GOS synthesis properties, its separation from the obtained reaction products is easy to accomplish
Subject: Tecnologia alimentar, Engenharia biológica, Biotecnologia alimentar, Outras ciências médicas
Food technology, Biological engineering, Food biotechnology, Other medical sciences
Scientific areas: Ciências médicas e da saúde::Outras ciências médicas
Medical and Health sciences::Other medical sciences
Source: Proceedings of the 10th International Chemical and Biological Engineering Conference
Document Type: Artigo em Livro de Atas de Conferência Internacional
Rights: restrictedAccess
Appears in Collections:FCNAUP - Artigo em Livro de Atas de Conferência Internacional

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