Please use this identifier to cite or link to this item: http://hdl.handle.net/10216/95287
Author(s): Rebeca Cruz
Eulalia Mendes
Ana Costa
Catarina Santos
Simone Morais
Susana Casal
Title: Validation of a Single-Extraction Procedure for Sequential Analysis of Vitamin E, Cholesterol, Fatty Acids, and Total Fat in Seafood
Issue Date: 2013
Abstract: Food lipid major components are usually analyzed by individual methodologies using diverse extractive procedures for each class. A simple and fast extractive procedure was devised for the sequential analysis of vitamin E, cholesterol, fatty acids, and total fat estimation in seafood, reducing analyses time and organic solvent consumption. Several liquid/liquid-based extractive methodologies using chlorinated and non-chlorinated organic solvents were tested. The extract obtained is used for vitamin E quantification (normal-phase HPLC with fluorescence detection), total cholesterol (normal-phase HPLC with UV detection), fatty acid profile, and total fat estimation (GC-FID), all accomplished in < 40 min. The final methodology presents an adequate linearity range and sensitivity for tocopherol and cholesterol, with intra- and inter-day precisions (RSD) from 3 to 11 % for all the components. The developed methodology was applied to diverse seafood samples with positive outcomes, making it a very attractive technique for routine analyses in standard equipped laboratories in the food quality control field.
Subject: Outras ciências agrárias
Other Agrarian Sciences
URI: http://hdl.handle.net/10216/95287
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FFUP - Artigo em Revista Científica Internacional

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