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Author(s): Lúcia Maia
Susana Casal
M. Beatriz Oliveira
Paula Oliveira
Title: Validation of a micromethod for quantification of lutein and beta-carotene in olive oil
Issue Date: 2008
Abstract: A micromethod was developed and validated for the quantification of lutein and beta-carotene in olive oils. The compounds are liquid-liquid extracted with n-hexane and n,n-dimethylformamide (DMF), by vortex mixing and centrifugation. The n-hexane phase retains beta-carotene, whereas the DMF phase retains lutein. This last fraction is concentrated and analyzed by RP-HPLC/diode-array, with a gradient of acetonitrile, water, and ethyl acetate, using beta-apo-8'-carotenal as I.S. beta-carotene is quantified by UV/Vis measurements at 454nm, by the external standard method. The method shows appropriate inter- and intra-day reproducibility, accuracy, and linearity for both compounds, allowing fast analysis of several samples simultaneously, with a relatively small consumption of organic solvents. The analytical methodology was applied to several samples of commercial Portuguese PDO olive oils.
Subject: Química
Chemical sciences
Scientific areas: Ciências exactas e naturais::Química
Natural sciences::Chemical sciences
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional
FFUP - Artigo em Revista Científica Internacional

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