Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/92557
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dc.creatorSantos, JM
dc.creatorBenite Ribeiro, SA
dc.creatorQueiroz, G
dc.creatorduarte, ja
dc.date.accessioned2019-05-21T23:11:14Z-
dc.date.available2019-05-21T23:11:14Z-
dc.date.issued2014
dc.identifier.issn0263-6484
dc.identifier.othersigarra:101654
dc.identifier.urihttps://hdl.handle.net/10216/92557-
dc.description.abstractContraction and insulin increase glucose uptake in skeletal muscle. While the insulin pathway, better characterized, requires activation of phosphoinositide 3-kinase (PI3K) and atypical protein kinase (aPKC), muscle contraction seems to share insulin-activated components to increase glucose uptake. This study aimed to investigate the interrelation between the pathway involved in glucose uptake evoked by insulin and muscle contraction. Isolated muscle of rats was treated with solvent (control), insulin, wortmannin (PI3K inhibitor) and the combination of insulin plus wortmannin. After treatment, muscles were electrically stimulated (contracted) or remained at rest. Glucose transporter 4 (GLUT4) localization, glucose uptake and phospho-aPKC (aPKC activated form) were assessed. Muscle contraction and insulin increased glucose uptake in all conditions when compared with controls not stimulating an effect that was accompanied by an increase in GLUT4 and of phospho-aPKC at the muscle membrane. Contracted muscles treated with insulin did not show additive effects on glucose uptake or aPKC activity compared with the response when these stimuli were applied alone. Inhibition of PI3K blocked insulin effect on glucose uptake and aPKC but not in the contractile response. Thus, muscle contraction seems to stimulate aPKC and glucose uptake independently of PI3K. Therefore, aPKC may be a convergence point and a rate limit step in the pathway by which, insulin and contraction, increase glucose uptake in skeletal muscle. Copyright (c) 2014 John Wiley & Sons, Ltd.
dc.language.isoeng
dc.rightsrestrictedAccess
dc.subjectCiências biológicas
dc.subjectBiological sciences
dc.titleThe interrelation between aPKC and glucose uptake in the skeletal muscle during contraction and insulin stimulation
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoFaculdade de Farmácia
dc.contributor.uportoFaculdade de Desporto
dc.identifier.doi10.1002/cbf.3081
dc.identifier.authenticusP-00A-3RA
dc.subject.fosCiências exactas e naturais::Ciências biológicas
dc.subject.fosNatural sciences::Biological sciences
Appears in Collections:FADEUP - Artigo em Revista Científica Internacional
FFUP - Artigo em Revista Científica Internacional

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