Computational Resources and Strategies to Construct Single-Molecule Models of FISH

Currently, the interactions occurring between oligonucleotides and the cellular envelope of bacteria are not fully resolved at the molecular level. Understanding these interactions is essential to gain insights on how to improve the internalization of the tagged oligonucleotides during fluorescence in situ hybridization (FISH). Agent-based modeling (ABM) is a promising in silico tool to dynamically simulate FISH and bring forward new knowledge on this process. Notably, it is important to simu...

Numerical Modeling of Bridge Piers Scouring Flow Patterns

Numerical models, compared to the physical models, provide real-scale predictions and are cost-effective. However, the reliability of the numerical results depends on how the governing equations are mathematically solved. This paper summarizes details of a numerical model, developed under the scope of the study of bridge piers local scour, which can be applied in different river engineering applications. The paper reports applications of the numerical model, namely the prediction of the turbu...

Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes

Oligonucleotides able to hybridize bacterial RNA via in situ hybridization may potentially act as new antimicrobials, replacing antibiotics, and as fast in vivo diagnostic probes, outperforming current clinical methodologies. Nonetheless, oligonucleotides are not able to efficiently permeate the multi-layered bacterial envelope to reach their target RNA in the cytosol. Cationic fusogenic liposomes are here suggested as vehicles to enable the internalization of oligonucleotides in bacteria. He...

An Introduction to Fluorescence in situ Hybridization in Microorganisms

Fluorescence in situ hybridization (FISH) is a molecular biology technique that enables the localization, quantification, and identification of microorganisms in a sample. This technique has found applications in several areas, most notably the environmental, for quantification and diversity assessment of microorganisms and, the clinical, for the rapid diagnostics of infectious agents. The FISH method is based on the hybridization of a fluorescently labeled nucleic acid probe with a complemen...