Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/130492
Author(s): | Pagels, F. Salvaterra, D. Amaro, H.M. Lopes, G. Sousa-Pinto, I. Vasconcelos, V. Guedes, A.C. |
Title: | Factorial optimization of upstream process for Cyanobium sp. pigments production |
Publisher: | Springer |
Issue Date: | 2020 |
Abstract: | Cyanobacteria-based pigments, such as carotenoids and phycobiliproteins, have emerged in the last few years as products with great economical interest. However, only the production of a few strains has been optimized for large-scale productions. As photosynthetic components, pigments have their synthesis modulated by abiotic factors, such as pH, temperature and salinity, which can lead to a huge impact on cyanobacteria production. This work aimed the optimization of biomass and pigments production by Cyanobium sp. LEGE 06113, using a factorial Box-Behnken design for three abiotic factors—temperature (20–30 °C), pH (6.0–9.0) and salinity (NaCl, 10–30 g L−1). Biomass, photosynthetic activity, carotenoid and phycobiliprotein productivity and antioxidant capacity of acetonic and aqueous extracts were measured over time and plotted into quadratic models. Results revealed that temperature and pH had a more significant impact than salinity on Cyanobium sp. metabolism and it was possible to determine a significant quadratic model for all evaluated parameters. According to the factorial modelling, the optimal condition for biomass, carotenoids and phycobiliprotein productivity was obtained at 20 °C, pH 9.0 and 10 g L−1 of NaCl, as subsequently confirmed in experimental trials, with an observed productivity of 127.12 ± 1.30 mgDW Lculture−1 day−1 for biomass; 2.04 ± 0.51 mgcarot Lculture−1 day−1 for total carotenoids; and 4.14 ± 0.71 mgphyco Lculture−1 day−1 for total phycobiliproteins. |
Subject: | Abiotic factors Antioxidant capacity Box-Behnken design Carotenoids Cyanobacteria Phycobiliproteins |
URI: | https://hdl.handle.net/10216/130492 |
Source: | Journal of Applied Phycology (2020) 32:3861–3872 |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | CIIMAR - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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Pagels_F_2020_3.pdf Restricted Access | 3.59 MB | Adobe PDF | Request a copy |
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