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dc.creatorPagels, F.
dc.creatorSalvaterra, D.
dc.creatorAmaro, H.M.
dc.creatorLopes, G.
dc.creatorSousa-Pinto, I.
dc.creatorVasconcelos, V.
dc.creatorGuedes, A.C.
dc.identifier.issnISSN 1573-5176
dc.description.abstractCyanobacteria-based pigments, such as carotenoids and phycobiliproteins, have emerged in the last few years as products with great economical interest. However, only the production of a few strains has been optimized for large-scale productions. As photosynthetic components, pigments have their synthesis modulated by abiotic factors, such as pH, temperature and salinity, which can lead to a huge impact on cyanobacteria production. This work aimed the optimization of biomass and pigments production by Cyanobium sp. LEGE 06113, using a factorial Box-Behnken design for three abiotic factors—temperature (20–30 °C), pH (6.0–9.0) and salinity (NaCl, 10–30 g L−1). Biomass, photosynthetic activity, carotenoid and phycobiliprotein productivity and antioxidant capacity of acetonic and aqueous extracts were measured over time and plotted into quadratic models. Results revealed that temperature and pH had a more significant impact than salinity on Cyanobium sp. metabolism and it was possible to determine a significant quadratic model for all evaluated parameters. According to the factorial modelling, the optimal condition for biomass, carotenoids and phycobiliprotein productivity was obtained at 20 °C, pH 9.0 and 10 g L−1 of NaCl, as subsequently confirmed in experimental trials, with an observed productivity of 127.12 ± 1.30 mgDW Lculture−1 day−1 for biomass; 2.04 ± 0.51 mgcarot Lculture−1 day−1 for total carotenoids; and 4.14 ± 0.71 mgphyco Lculture−1 day−1 for total phycobiliproteins.
dc.description.sponsorshipThis work was co-funded by the national founding from FCT (UIDB/04423/2020 and UIDP/04423/2020); by the Atlantic Interreg Projects, Enhance MicroAlgae, high added value industrial opportunities for microalgae in the Atlantic Area (EAPA_338/2016), and by the BLUEHUMAN, BLUE biotechnology as a road for innovation on human’s health aiming smart growth in Atlantic Area (EAPA_151/2016).
dc.relation.ispartofJournal of Applied Phycology (2020) 32:3861–3872
dc.subjectAbiotic factors
dc.subjectAntioxidant capacity
dc.subjectBox-Behnken design
dc.titleFactorial optimization of upstream process for Cyanobium sp. pigments production
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoCentro Interdisciplinar de Investigação Marinha e Ambiental
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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