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Author(s): Gomes, CP
Oliveira, H
Ebner, A
Hinterdorfer, P
Pêgo, AP
Title: Molecular Recognition Force Spectroscopy for Probing Cell Targeted Nanoparticles In Vitro
Publisher: Humana Press
Issue Date: 2018-10-30
Description: In the development and design of cell targeted nanoparticle-based systems the density of targeting moieties plays a fundamental role in allowing maximal cell-specific interaction. Here, we describe the use of molecular recognition force spectroscopy as a valuable tool for the characterization and optimization of targeted nanoparticles toward attaining cell-specific interaction. By tailoring the density of targeting moieties at the nanoparticle surface, one can correlate the unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and cells to the nanoparticle vectoring capacity. This novel approach allows for a rapid and cost-effective design of targeted nanomedicines reducing the need for long and tedious in vitro tests.
Subject: Animals
Cell Line
Data Analysis
Drug Delivery Systems
Microscopy, Atomic Force / methods
Molecular Imaging / methods
Molecular Probes
Nanoparticles / chemistry
Single Molecule Imaging / methods
Series: Methods in molecular biology, vol. 1886, p. 327-341
Related Information: info:eu-repo/grantAgreement/FCT/5876/147342/PT
Document Type: Capítulo ou Parte de Livro
Rights: openAccess
Appears in Collections:I3S - Capítulo ou Parte de Livro

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