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Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/126850
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dc.creatorGomes, CP-
dc.creatorOliveira, H-
dc.creatorEbner, A-
dc.creatorHinterdorfer, P-
dc.creatorPêgo, AP-
dc.date.accessioned2020-04-03T16:26:09Z-
dc.date.available2020-04-03T16:26:09Z-
dc.date.issued2018-10-30-
dc.identifier.issn1064-3745-
dc.identifier.urihttps://hdl.handle.net/10216/126850-
dc.descriptionIn the development and design of cell targeted nanoparticle-based systems the density of targeting moieties plays a fundamental role in allowing maximal cell-specific interaction. Here, we describe the use of molecular recognition force spectroscopy as a valuable tool for the characterization and optimization of targeted nanoparticles toward attaining cell-specific interaction. By tailoring the density of targeting moieties at the nanoparticle surface, one can correlate the unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and cells to the nanoparticle vectoring capacity. This novel approach allows for a rapid and cost-effective design of targeted nanomedicines reducing the need for long and tedious in vitro tests.pt_PT
dc.description.sponsorshipThe authors would like to acknowledge the Bioimaging Platform (i3S-INEB) for the support with atomic force microscopy. This work was financed by projects NORTE-01-0145-FEDER-000008 and NORTE-01-0145-FEDER-000012, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020; and by Portuguese funds through FCT (Fundação para a Ciência e a Tecnologia) in the framework of the projects UID/BIM/04293/ 2013, PTDC/CTM-NAN/115124/2009, and PTDC/CTMNAN/3547/2014. C.P. Gomes acknowledge FCT for her PhD scholarship SFRH/BD/79930/2011.pt_PT
dc.language.isoengpt_PT
dc.publisherHumana Presspt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147342/PT-
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/115124/PT-
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F79930%2F2011/PT-
dc.relation.ispartofseriesMethods in molecular biology, vol. 1886, p. 327-341pt_PT
dc.rightsopenAccesspt_PT
dc.subjectAnimalspt_PT
dc.subjectCell Linept_PT
dc.subjectData Analysispt_PT
dc.subjectDrug Delivery Systemspt_PT
dc.subjectHumanspt_PT
dc.subjectMicept_PT
dc.subjectMicroscopy, Atomic Force / methodspt_PT
dc.subjectMolecular Imaging / methodspt_PT
dc.subjectMolecular Probespt_PT
dc.subjectNanomedicinept_PT
dc.subjectNanoparticles / chemistrypt_PT
dc.subjectSingle Molecule Imaging / methodspt_PT
dc.titleMolecular Recognition Force Spectroscopy for Probing Cell Targeted Nanoparticles In Vitropt_PT
dc.typeCapítulo ou Parte de Livropt_PT
dc.contributor.uportoInstituto de Investigação e Inovação em Saúdept_PT
dc.identifier.doi10.1007/978-1-4939-8894-5_19-
dc.relation.publisherversionhttps://link.springer.com/protocol/10.1007%2F978-1-4939-8894-5_19-
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