Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120488
Author(s): Machado A.M.
Tørresen O.K.
Kabeya N.
Couto A.
Petersen B.
Felício M.
Campos P.F.
Fonseca E.
Bandarra N.
Lopes-Marques M.
Ferraz R.
Ruivo R.
Fonseca M.M.
Jentoft S.
Monroig Ó.
da Fonseca R.R.
Castro L.F.C.
Title: “Out of the Can”: A Draft Genome Assembly, Liver Transcriptome, and Nutrigenomics of the European Sardine, Sardina pilchardus
Publisher: MDPI
Issue Date: 2018
Abstract: Clupeiformes, such as sardines and herrings, represent an important share of worldwide fisheries. Among those, the European sardine (Sardina pilchardus, Walbaum 1792) exhibits significant commercial relevance. While the last decade showed a steady and sharp decline in capture levels, recent advances in culture husbandry represent promising research avenues. Yet, the complete absence of genomic resources from sardine imposes a severe bottleneck to understand its physiological and ecological requirements. We generated 69 Gbp of paired-end reads using Illumina HiSeq X Ten and assembled a draft genome assembly with an N50 scaffold length of 25,579 bp and BUSCO completeness of 82.1% (Actinopterygii). The estimated size of the genome ranges between 655 and 850 Mb. Additionally, we generated a relatively high-level liver transcriptome. To deliver a proof of principle of the value of this dataset, we established the presence and function of enzymes (Elovl2, Elovl5, and Fads2) that have pivotal roles in the biosynthesis of long chain polyunsaturated fatty acids, essential nutrients particularly abundant in oily fish such as sardines. Our study provides the first sustainableomics datasetexploitation.from a valuable economic marine teleost species, the European sardine, representing an essential resource for their effective conservation, management, and sustainable exploitation. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
Subject: polyunsaturated fatty acid
transcriptome
animal tissue
Article
biosynthesis
genome
genome size
liver
marine species
nonhuman
nutrigenomics
phylogenomics
phylogeny
Sardina pilchardus
sardine
species conservation
species diversity
teleost
URI: https://hdl.handle.net/10216/120488
Source: Genes, vol. 9(10):485
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

Files in This Item:
File Description SizeFormat 
Machado AM_4_2018.pdf2.04 MBAdobe PDFThumbnail
View/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.