Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120473
Author(s): Kumar J.G.S.P.
Gomathi A.
Gothandam K.M.
Vasconcelos V.
Title: Bioactivity assessment of indian origin-mangrove actinobacteria against candida albicans
Publisher: MDPI
Issue Date: 2018
Abstract: Actinobacteria is found to have a potent metabolic activity against pathogens. The present study reveals the assessment of potent antifungal secondary metabolites from actinobacteria isolated from Indian marine mangrove sediments. The samples were collected from the coastal regions of Muthupet, Andaman and the Nicobar Islands. Identification was carried out using 16S rRNA analysis and biosynthetic genes (Polyketide synthase type I/II and Non-ribosomal peptide synthase) were screened. Actinobacteria were assayed for their antifungal activity against 16 clinical Candida albicans and the compound analysis was performed using gas chromatography-mass spectrometry GC-MS. The 31 actinobacterial strains were isolated and 16S rRNA gene sequencing revealed that this ecosystem is rich on actinobacteria, with Streptomyces as the predominant genus. The PCR based screening of biosynthetic genes revealed the presence of PKS-I in six strains, PKS-II in four strains and NRPS in 11 strains. The isolated actinobacteria VITGAP240 and VITGAP241 (two isolates) were found to have a potential antifungal activity against all the tested C. albicans. GC-MS results revealed that the actinobacterial compounds were belonging to heterocyclic, polyketides and peptides. Overall, the strains possess a wide spectrum of antifungal properties which affords the production of significant bioactive metabolites as potential antibiotics. © 2018 by the authors.
Subject: Actinobacteria extract
antibiotic agent
antifungal agent
nonribosomal peptide synthetase
polyketide synthase
polyketide synthase type I
polyketide synthase type II
RNA 16S
unclassified drug
antifungal agent
RNA 16S
Actinobacteria
Andaman and Nicobar Islands
antifungal activity
Article
bacterial strain
bacterium identification
bacterium isolate
Candida albicans
controlled study
drug isolation
drug screening
drug synthesis
ecosystem
gene sequence
genetic screening
mangrove
mass fragmentography
metabolite
nonhuman
nucleotide sequence
phylogeny
RNA analysis
sediment
Streptomyces
Actinobacteria
Candida albicans
chemistry
drug effect
genetics
India
microbial sensitivity test
microbiology
Rhizophoraceae
Actinobacteria
Antifungal Agents
Candida albicans
Gas Chromatography-Mass Spectrometry
Geologic Sediments
India
Microbial Sensitivity Tests
Rhizophoraceae
RNA, Ribosomal, 16S
Streptomyces
URI: https://hdl.handle.net/10216/120473
Source: Marine Drugs, vol. 16(2):60
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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