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https://hdl.handle.net/10216/120473
Autor(es): | Kumar J.G.S.P. Gomathi A. Gothandam K.M. Vasconcelos V. |
Título: | Bioactivity assessment of indian origin-mangrove actinobacteria against candida albicans |
Editor: | MDPI |
Data de publicação: | 2018 |
Resumo: | Actinobacteria is found to have a potent metabolic activity against pathogens. The present study reveals the assessment of potent antifungal secondary metabolites from actinobacteria isolated from Indian marine mangrove sediments. The samples were collected from the coastal regions of Muthupet, Andaman and the Nicobar Islands. Identification was carried out using 16S rRNA analysis and biosynthetic genes (Polyketide synthase type I/II and Non-ribosomal peptide synthase) were screened. Actinobacteria were assayed for their antifungal activity against 16 clinical Candida albicans and the compound analysis was performed using gas chromatography-mass spectrometry GC-MS. The 31 actinobacterial strains were isolated and 16S rRNA gene sequencing revealed that this ecosystem is rich on actinobacteria, with Streptomyces as the predominant genus. The PCR based screening of biosynthetic genes revealed the presence of PKS-I in six strains, PKS-II in four strains and NRPS in 11 strains. The isolated actinobacteria VITGAP240 and VITGAP241 (two isolates) were found to have a potential antifungal activity against all the tested C. albicans. GC-MS results revealed that the actinobacterial compounds were belonging to heterocyclic, polyketides and peptides. Overall, the strains possess a wide spectrum of antifungal properties which affords the production of significant bioactive metabolites as potential antibiotics. © 2018 by the authors. |
Assunto: | Actinobacteria extract antibiotic agent antifungal agent nonribosomal peptide synthetase polyketide synthase polyketide synthase type I polyketide synthase type II RNA 16S unclassified drug antifungal agent RNA 16S Actinobacteria Andaman and Nicobar Islands antifungal activity Article bacterial strain bacterium identification bacterium isolate Candida albicans controlled study drug isolation drug screening drug synthesis ecosystem gene sequence genetic screening mangrove mass fragmentography metabolite nonhuman nucleotide sequence phylogeny RNA analysis sediment Streptomyces Actinobacteria Candida albicans chemistry drug effect genetics India microbial sensitivity test microbiology Rhizophoraceae Actinobacteria Antifungal Agents Candida albicans Gas Chromatography-Mass Spectrometry Geologic Sediments India Microbial Sensitivity Tests Rhizophoraceae RNA, Ribosomal, 16S Streptomyces |
URI: | https://hdl.handle.net/10216/120473 |
Fonte: | Marine Drugs, vol. 16(2):60 |
Informação Relacionada: | info:eu-repo/grantAgreement/FCT/5876/147268/PT |
Tipo de Documento: | Artigo em Revista Científica Internacional |
Condições de Acesso: | openAccess |
Aparece nas coleções: | CIIMAR - Artigo em Revista Científica Internacional |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Kumar JGSP_2018.pdf | 7.27 MB | Adobe PDF | Ver/Abrir |
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