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Author(s): Frazão B.
Antunes A.
Title: Jellyfish bioactive compounds: Methods for wet-lab work
Publisher: MDPI
Issue Date: 2016
Abstract: The study of bioactive compounds from marine animals has provided, over time, an endless source of interesting molecules. Jellyfish are commonly targets of study due to their toxic proteins. However, there is a gap in reviewing successful wet-lab methods employed in these animals, which compromises the fast progress in the detection of related biomolecules. Here, we provide a compilation of the most effective wet-lab methodologies for jellyfish venom extraction prior to proteomic analysis-separation, identification and toxicity assays. This includes SDS-PAGE, 2DE, gel chromatography, HPLC, DEAE, LC-MS, MALDI, Western blot, hemolytic assay, antimicrobial assay and protease activity assay. For a more comprehensive approach, jellyfish toxicity studies should further consider transcriptome sequencing. We reviewed such methodologies and other genomic techniques used prior to the deep sequencing of transcripts, including RNA extraction, construction of cDNA libraries and RACE. Overall, we provide an overview of the most promising methods and their successful implementation for optimizing time and effort when studying jellyfish. © 2016 by the authors; licensee MDPI, Basel, Switzerland.
Subject: 2 diethylaminoethanol
complementary DNA
fish venom
jellyfish venom
unclassified drug
amino acid sequence
antimicrobial assay
cell disruption
chemical analysis
DNA library
drug determination
enzyme assay
fast flow anion exchange chromatography
fast protein liquid chromatography
gene sequence
genetic trait
genetic transcription
high performance liquid chromatography
liquid chromatography
mass spectrometry
matrix assisted laser desorption ionization time of flight mass spectrometry
mitochondrial genome
next generation sequencing
polyacrylamide gel electrophoresis
posttranscriptional gene silencing
protein degradation
protein determination
RNA extraction
RNA transcription
separation technique
single nucleotide polymorphism
size exclusion chromatography
toxicity testing
two dimensional electrophoresis
Western blotting
wet lab method
Source: Marine Drugs, vol. 14(4):75
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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