Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/120452
Author(s): | Frazão B. Antunes A. |
Title: | Jellyfish bioactive compounds: Methods for wet-lab work |
Publisher: | MDPI |
Issue Date: | 2016 |
Abstract: | The study of bioactive compounds from marine animals has provided, over time, an endless source of interesting molecules. Jellyfish are commonly targets of study due to their toxic proteins. However, there is a gap in reviewing successful wet-lab methods employed in these animals, which compromises the fast progress in the detection of related biomolecules. Here, we provide a compilation of the most effective wet-lab methodologies for jellyfish venom extraction prior to proteomic analysis-separation, identification and toxicity assays. This includes SDS-PAGE, 2DE, gel chromatography, HPLC, DEAE, LC-MS, MALDI, Western blot, hemolytic assay, antimicrobial assay and protease activity assay. For a more comprehensive approach, jellyfish toxicity studies should further consider transcriptome sequencing. We reviewed such methodologies and other genomic techniques used prior to the deep sequencing of transcripts, including RNA extraction, construction of cDNA libraries and RACE. Overall, we provide an overview of the most promising methods and their successful implementation for optimizing time and effort when studying jellyfish. © 2016 by the authors; licensee MDPI, Basel, Switzerland. |
Subject: | 2 diethylaminoethanol complementary DNA fish venom glycoprotein jellyfish venom phosphoprotein proteinase transcriptome unclassified drug amino acid sequence antimicrobial assay autolysis cell disruption chemical analysis DNA library drug determination electrophoresis enzyme assay fast flow anion exchange chromatography fast protein liquid chromatography gene sequence genetic trait genetic transcription genomics hemolysis high performance liquid chromatography jellyfish liquid chromatography mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry mitochondrial genome nematocyst next generation sequencing nonhuman polyacrylamide gel electrophoresis posttranscriptional gene silencing protein degradation protein determination proteomics Review RNA extraction RNA transcription separation technique single nucleotide polymorphism size exclusion chromatography toxicity testing transcriptomics two dimensional electrophoresis ultrasound Western blotting wet lab method |
URI: | https://hdl.handle.net/10216/120452 |
Source: | Marine Drugs, vol. 14(4):75 |
Related Information: | info:eu-repo/grantAgreement/FCT/5876/147268/PT |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | openAccess |
Appears in Collections: | CIIMAR - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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Frazao B_2016.pdf | 4.33 MB | Adobe PDF | View/Open |
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