Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120414
Author(s): Pratheepa V.
Alex A.
Silva M.
Vasconcelos V.
Title: Bacterial diversity and tetrodotoxin analysis in the viscera of the gastropods from Portuguese coast
Publisher: Elsevier
Issue Date: 2016
Abstract: To trace the pathway of tetrodotoxin (TTX) producing microorganism in the Atlantic coast of Portugal, culture-dependent evaluation of the bacterial isolates from the viscera of the gastropods Monodonta lineata, Gibbula umbilicalis, Nucella lapillus and Patella intermedia, and from the environmental samples (biofilm and surrounding sea water) was carried out. Samples were collected from eight different coastal locations of Northern Portugal. A total of 311 isolates were identified. The observed bacterial diversity was distributed over five different classes (Gammaproteobacteria, Alphaproteobacteria, Flavobacteria, Bacilli and Actinobacteria) with the greatest number of 16S rRNA gene sequence derived from the Gammaproteobacteria (75%). Phylogenetic analysis based on the 16S rRNA gene showed that bacterial isolates were highly diverse and most of which were found in other marine environment. Among the different species isolated, Vibrio was found abundant. Eventhough TTX was not detected (UPLC-MS/MS) in the isolates from this study, PCR screening identified some natural product biosynthesis genes (PKS and NRPS) involved in its assembly. Further PCR screening of the TTX producing two ATCC Vibrio sp. reveals that NRPS might be involved in the biosynthesis of TTX through the incorporation of arginine. © 2016 Elsevier.
Subject: RNA 16S
sea water
tetrodotoxin
Actinobacteria
Alphaproteobacteria
AMT gene
Article
Bacilli
bacterial gene
bacterium culture
bacterium identification
bacterium isolate
bacterium isolation
coastal waters
controlled study
Flavobacteria
Gammaproteobacteria
gastropod
gene sequence
Gibbula umbilicalis
marine environment
microbial diversity
molecular phylogeny
Monodonta lineata
nonhuman
NRPS gene
Nucella lapillus
Patella intermedia
PKS gene
polymerase chain reaction
population abundance
Portugal
priority journal
tandem mass spectrometry
toxin analysis
ultra performance liquid chromatography
water sampling
URI: https://hdl.handle.net/10216/120414
Source: Toxicon, vol. 119, p. 186-193
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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