Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120414
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dc.creatorPratheepa V.
dc.creatorAlex A.
dc.creatorSilva M.
dc.creatorVasconcelos V.
dc.date.accessioned2019-05-31T16:15:32Z-
dc.date.available2019-05-31T16:15:32Z-
dc.date.issued2016
dc.identifier.issn00410101
dc.identifier.urihttps://hdl.handle.net/10216/120414-
dc.description.abstractTo trace the pathway of tetrodotoxin (TTX) producing microorganism in the Atlantic coast of Portugal, culture-dependent evaluation of the bacterial isolates from the viscera of the gastropods Monodonta lineata, Gibbula umbilicalis, Nucella lapillus and Patella intermedia, and from the environmental samples (biofilm and surrounding sea water) was carried out. Samples were collected from eight different coastal locations of Northern Portugal. A total of 311 isolates were identified. The observed bacterial diversity was distributed over five different classes (Gammaproteobacteria, Alphaproteobacteria, Flavobacteria, Bacilli and Actinobacteria) with the greatest number of 16S rRNA gene sequence derived from the Gammaproteobacteria (75%). Phylogenetic analysis based on the 16S rRNA gene showed that bacterial isolates were highly diverse and most of which were found in other marine environment. Among the different species isolated, Vibrio was found abundant. Eventhough TTX was not detected (UPLC-MS/MS) in the isolates from this study, PCR screening identified some natural product biosynthesis genes (PKS and NRPS) involved in its assembly. Further PCR screening of the TTX producing two ATCC Vibrio sp. reveals that NRPS might be involved in the biosynthesis of TTX through the incorporation of arginine. © 2016 Elsevier.
dc.description.sponsorshipThis research was partially supported by the European Regional Development Fund (ERDF) through the COMPETE Operational Competitiveness Programme and national funds through FCT Foundation for Science and Technology under the project UID/Multi/04423/2013 and by the Structured Program of R&D&I INNOVMAR - Innovation and Sustainability in the Management and Exploitation of Marine Resources (reference NORTE-01-0145-FEDER-000035, Research Line NOVELMAR), funded by the Northern Regional Operational Programme (NORTE2020) through the European Regional Development Fund (ERDF). PV also acknowledges FCT for postdoctoral grant (SFRH/BPD/78269/2011), Joana Azevedo and Micaela Vale for the timely support. Also acknowledges CIFGA, Spain for the UPLC-MS/MS analysis.
dc.language.isoeng
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147268/PT
dc.relation.ispartofToxicon, vol. 119, p. 186-193
dc.rightsrestrictedAccess
dc.subjectRNA 16S
dc.subjectsea water
dc.subjecttetrodotoxin
dc.subjectActinobacteria
dc.subjectAlphaproteobacteria
dc.subjectAMT gene
dc.subjectArticle
dc.subjectBacilli
dc.subjectbacterial gene
dc.subjectbacterium culture
dc.subjectbacterium identification
dc.subjectbacterium isolate
dc.subjectbacterium isolation
dc.subjectcoastal waters
dc.subjectcontrolled study
dc.subjectFlavobacteria
dc.subjectGammaproteobacteria
dc.subjectgastropod
dc.subjectgene sequence
dc.subjectGibbula umbilicalis
dc.subjectmarine environment
dc.subjectmicrobial diversity
dc.subjectmolecular phylogeny
dc.subjectMonodonta lineata
dc.subjectnonhuman
dc.subjectNRPS gene
dc.subjectNucella lapillus
dc.subjectPatella intermedia
dc.subjectPKS gene
dc.subjectpolymerase chain reaction
dc.subjectpopulation abundance
dc.subjectPortugal
dc.subjectpriority journal
dc.subjecttandem mass spectrometry
dc.subjecttoxin analysis
dc.subjectultra performance liquid chromatography
dc.subjectwater sampling
dc.titleBacterial diversity and tetrodotoxin analysis in the viscera of the gastropods from Portuguese coast
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoCIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental
dc.identifier.doi10.1016/j.toxicon.2016.06.003
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.toxicon.2016.06.003
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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