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Author(s): Domínguez-Pérez D.
Rodríguez A.A.
Osorio H.
Azevedo J.
Castañeda O.
Vasconcelos V.
Antunes A.
Title: Microcystin-lr detected in a low molecular weight fraction from a crude extract of Zoanthus sociatus
Publisher: MDPI
Issue Date: 2017
Abstract: Cnidarian constitutes a great source of bioactive compounds. However, research involving peptides from organisms belonging to the order Zoanthidea has received very little attention, contrasting to the numerous studies of the order Actiniaria, from which hundreds of toxic peptides and proteins have been reported. In this work, we performed a mass spectrometry analysis of a low molecular weight (LMW) fraction previously reported as lethal to mice. The low molecular weight (LMW) fraction was obtained by gel filtration of a Zoanthus sociatus (order Zoanthidea) crude extract with a Sephadex G-50, and then analyzed by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometry (MS) in positive ion reflector mode from m/z 700 to m/z 4000. Afterwards, some of the most intense and representative MS ions were fragmented by MS/MS with no significant results obtained by Protein Pilot protein identification software and the Mascot algorithm search. However, microcystin masses were detected by mass-matching against libraries of non-ribosomal peptide database (NORINE). Subsequent reversed-phase C18 HPLC (in isocratic elution mode) and mass spectrometry analyses corroborated the presence of the cyanotoxin Microcystin-LR (MC-LR). To the best of our knowledge, this finding constitutes the first report of MC-LR in Z. sociatus, and one of the few evidences of such cyanotoxin in cnidarians. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.
Subject: microcystin
peptide synthase
microcystin LR
cell viability
controlled study
food contamination
gel filtration
liquid chromatography
marine environment
mass spectrometry
matrix assisted laser desorption ionization time of flight mass spectrometry
molecular weight
protein analysis
signal detection
Zoanthus sociatus
drug mixture
molecular weight
Complex Mixtures
Molecular Weight
Source: Toxins, vol. 9(3):89
Related Information: info:eu-repo/grantAgreement/FCT/5876/147268/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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