Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/120386
Author(s): | Domínguez-Pérez D. Rodríguez A.A. Osorio H. Azevedo J. Castañeda O. Vasconcelos V. Antunes A. |
Title: | Microcystin-lr detected in a low molecular weight fraction from a crude extract of Zoanthus sociatus |
Publisher: | MDPI |
Issue Date: | 2017 |
Abstract: | Cnidarian constitutes a great source of bioactive compounds. However, research involving peptides from organisms belonging to the order Zoanthidea has received very little attention, contrasting to the numerous studies of the order Actiniaria, from which hundreds of toxic peptides and proteins have been reported. In this work, we performed a mass spectrometry analysis of a low molecular weight (LMW) fraction previously reported as lethal to mice. The low molecular weight (LMW) fraction was obtained by gel filtration of a Zoanthus sociatus (order Zoanthidea) crude extract with a Sephadex G-50, and then analyzed by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometry (MS) in positive ion reflector mode from m/z 700 to m/z 4000. Afterwards, some of the most intense and representative MS ions were fragmented by MS/MS with no significant results obtained by Protein Pilot protein identification software and the Mascot algorithm search. However, microcystin masses were detected by mass-matching against libraries of non-ribosomal peptide database (NORINE). Subsequent reversed-phase C18 HPLC (in isocratic elution mode) and mass spectrometry analyses corroborated the presence of the cyanotoxin Microcystin-LR (MC-LR). To the best of our knowledge, this finding constitutes the first report of MC-LR in Z. sociatus, and one of the few evidences of such cyanotoxin in cnidarians. © 2017 by the authors. Licensee MDPI, Basel, Switzerland. |
Subject: | microcystin peptide synthase sephadex microcystin microcystin LR Article cardiotoxicity cell viability Cnidaria controlled study cyanobacterium food contamination gel filtration liquid chromatography marine environment mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry molecular weight mouse nonhuman protein analysis signal detection Zoanthus sociatus analysis animal Anthozoa chemistry drug mixture molecular weight Animals Anthozoa Complex Mixtures Microcystins Molecular Weight |
URI: | https://hdl.handle.net/10216/120386 |
Source: | Toxins, vol. 9(3):89 |
Related Information: | info:eu-repo/grantAgreement/FCT/5876/147268/PT |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | openAccess |
Appears in Collections: | CIIMAR - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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Dominguez-Perez D_2017.pdf | 2.55 MB | Adobe PDF | View/Open |
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