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https://hdl.handle.net/10216/120347
Author(s): | Guzmán-Guillén R. Prieto Ortega A.I. Moyano R. Blanco A. Vasconcelos V. Cameán A.M. |
Title: | Dietary l-carnitine prevents histopathological changes in tilapia (Oreochromis Niloticus) exposed to cylindrospermopsin |
Publisher: | John Wiley and Sons |
Issue Date: | 2017 |
Abstract: | Cylindrospermopsin (CYN) is a cytotoxin highly water-soluble, which is easily taken up by several aquatic organisms. CYN acts as a potent protein and glutathione synthesis inhibitor, as well as inducing genotoxicity, oxidative stress, and histopathological alterations. This is the first study reporting the protective effect of a l-carnitine (LC) pretreatment (400 or 880 mg LC/kg bw fish/day, for 21 days) on the histopathological alterations induced by pure CYN or Aphanizomenon ovalisporum lyophilized cells (400 µg CYN/kg bw fish) in liver, kidney, heart, intestines, and gills of tilapia (Oreochromis niloticus) acutely exposed to the toxin by oral route. The main histopathological changes induced by CYN were disorganized parenchyma with presence of glycogen and lipids in the cytoplasm (liver), glomerulonephritis, glomerular atrophy, and dilatation of Bowman's capsule (kidney), myofibrolysis, loss of myofibrils, with edema and hemorrhage (heart), intestinal villi with necrotic enterocytes and partial loss of microvilli (gastrointestinal tract), and hyperemia and hemorrhage (gills). LC pretreatment was able to totally prevent those CYN-induced alterations from 400 mg LC/kg bw fish/day in almost all organs, except in the heart, where 880 mg LC/kg bw fish/day were needed. In addition, the morphometric study indicated that LC managed to recover totally the affectation in the cross sections of the proximal and distal convoluted tubules in CYN-exposed fish. © 2015 Wiley Periodicals, Environ Toxicol 32: 241–254, 2017. © 2015 Wiley Periodicals, |
Subject: | Aquatic organisms Biosynthesis Cytology Aphanizomenon ovalisporum Cylindrospermopsin Histopathology L-carnitine Tilapia Fish carnitine cylindrospermopsin glycogen lipid bacterial toxin carnitine cylindrospermopsin protective agent uracil water pollutant cichlid cyanobacterium cytology cytoplasm diet genotoxicity histopathology inhibitor morphometry oxidative stress protein toxin animal experiment animal tissue antioxidant activity Article bleeding Bowman capsule controlled study cytoplasm diet supplementation freeze drying genotoxicity gill glomerulonephritis glutathione metabolism heart histopathology hyperemia intestine intestine cell kidney kidney distal tubule liver muscle fibril nonhuman Oreochromis niloticus oxidative stress priority journal analogs and derivatives animal Aphanizomenon cardiac muscle cichlid diet drug effects electron microscopy metabolism oxidative stress pathology toxicity water pollutant Aphanizomenon ovalisporum Oreochromis niloticus Tilapia Animals Aphanizomenon Bacterial Toxins Carnitine Cichlids Diet Gills Heart Kidney Liver Microscopy, Electron Myocardium Oxidative Stress Protective Agents Uracil Water Pollutants |
URI: | https://hdl.handle.net/10216/120347 |
Source: | Environmental Toxicology, vol. 32(1), p. 241-254 |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | CIIMAR - Artigo em Revista Científica Internacional |
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