Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120272
Author(s): André A.
Ruivo R.
Capitão A.
Froufe E.
Páscoa I.
Costa Castro L.F.
Santos M.M.
Title: Cloning and functional characterization of a retinoid X receptor orthologue in Platynereis dumerilii: An evolutionary and toxicological perspective
Publisher: Elsevier
Issue Date: 2017
Abstract: In the present work we provide the first isolation and functional characterization of a RXR orthologue in an annelid species, the Platynereis dumerilii. Using an in vitro luciferase reporter assay we evaluated the annelid receptor ability to respond to ligand 9-cis-retinoic acid, TBT and TPT. Our results show that the annelid RXR responds to 9-cis-retinoic acid and to the organotins by activating reporter gene transcription. The findings suggest a conserved mode of action of the receptor in response to a common signaling molecule and modulation by organotin compounds between vertebrates and Lophotrochozoans. © 2017 Elsevier
Subject: Organometallics
Annelid
Endocrine disruption
Evolution
Retinoid X receptor
Tributyltin
Triphenyltin
Transcription
retinoic acid
retinoid X receptor
alitretinoin
ligand
organotin compound
retinoid X receptor
activation energy
annelid
bioassay
endocrine disruptor
evolution
genetic analysis
laboratory method
ligand
organic acid
toxicology
Article
in vitro study
luciferase assay
molecular cloning
nonhuman
Platynereis dumerilii
Polychaeta
reporter gene
animal
drug effects
evolution
gene expression regulation
genetics
metabolism
physiology
Polychaeta
transcription initiation
Annelida
Lophotrochozoa
Platynereis dumerilii
Vertebrata
Animals
Biological Evolution
Gene Expression Regulation
Ligands
Organotin Compounds
Polychaeta
Retinoid X Receptors
Transcriptional Activation
Tretinoin
URI: https://hdl.handle.net/10216/120272
Source: Chemosphere, vol. 182, p. 753-761
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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