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Author(s): Balmaña, M
Mereiter, S
Diniz, F
Feijão, T
Barrias, CC
Reis, CA
Title: Multicellular Human Gastric Cancer Spheroids Mimic the Glycosylation Phenotype of Gastric Carcinomas
Publisher: MDPI
Issue Date: 2018
Abstract: Cellular glycosylation plays a pivotal role in several molecular mechanisms controlling cell⁻cell recognition, communication, and adhesion. Thus, aberrant glycosylation has a major impact on the acquisition of malignant features in the tumor progression of patients. To mimic these in vivo features, an innovative high-throughput 3D spheroid culture methodology has been developed for gastric cancer cells. The assessment of cancer cell spheroids' physical characteristics, such as size, morphology and solidity, as well as the impact of glycosylation inhibitors on spheroid formation was performed applying automated image analysis. A detailed evaluation of key glycans and glycoproteins displayed by the gastric cancer spheroids and their counterpart cells cultured under conventional 2D conditions was performed. Our results show that, by applying 3D cell culture approaches, the model cell lines represented the differentiation features observed in the original tumors and the cellular glycocalix underwent striking changes, displaying increased expression of cancer-associated glycan antigens and mucin MUC1, ultimately better simulating the glycosylation phenotype of the gastric tumor.
Subject: Carcinoma/genetics
Cell Communication/genetics
Cell Culture Techniques/methods
Cell Differentiation/genetics
Cell Line, Tumor
Spheroids, Cellular/metabolism
Spheroids, Cellular/pathology
Stomach Neoplasms/metabolism
Stomach Neoplasms/pathology
Series: Molecules, vol 23(11), pii: E2815
Related Information: info:eu-repo/grantAgreement/EC/H2020/748880/EU
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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