Please use this identifier to cite or link to this item: https://repositorio-aberto.up.pt/handle/10216/118214
Author(s): Dias, A
Almeida, CR
Reis, CA
Pinho, SS
Title: Studying T Cells N-Glycosylation by Imaging Flow Cytometry
Publisher: Humana Press
Issue Date: 2016
Abstract: Imaging flow cytometry is an emerging imaging technology that combines features of both conventional flow cytometry and fluorescence microscopy allowing quantification of the imaging parameters. The analysis of protein posttranslational modifications by glycosylation using imaging flow cytometry constitutes an important bioimaging tool in the glycobiology field. This technique allows quantification of the glycan fluorescence intensity, co-localization with proteins, and evaluation of the membrane/cytoplasmic expression. In this chapter we provide the guidelines to analyze glycan expression, particularly the ß1,6 GlcNAc branched N-glycans, on the membrane of intestinal T cells from inflammatory bowel disease patients.
Subject: Colitis, Ulcerative/immunology
Colitis, Ulcerative/pathology
Flow Cytometry/methods
Glycosylation
Humans
Image Cytometry
Intestines/immunology
Intestines/pathology
Microscopy, Fluorescence/methods
Plant Lectins/immunology
Polysaccharides/analysis
Polysaccharides/metabolism
T-Lymphocytes/metabolism
T-Lymphocytes/pathology
URI: https://repositorio-aberto.up.pt/handle/10216/118214
Source: Methods in Molecular Biology, vol.1389, p. 167-176
Related Information: info:eu-repo/grantAgreement/FCT/COMPETE/125428/PT
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/124445/PT
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F63094%2F2009/PT
info:eu-repo/grantAgreement/FCT/PD/PD%2FBD%2F105982%2F2014/PT
Document Type: Capítulo ou Parte de Livro
Rights: openAccess
Appears in Collections:I3S - Capítulo ou Parte de Livro

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