Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/118191
Author(s): | Barros, D Lima, S Cordeiro-da-Silva, A |
Title: | Surface functionalization of polymeric nanospheres modulates macrophage activation: Relevance in Leishmaniasis therapy |
Publisher: | Future Medicine |
Issue Date: | 2015 |
Abstract: | To characterize the production and application of carbohydrate functionalized poly(d,l-lactide-co-glycolide) (PLGA) nanospheres as immune-modulatory mediators in the treatment of visceral leishmaniasis (VL). Materials & methods: PLGA nanospheres were prepared by nanoprecipitation and surface functionalized with mannose, mannan or mannosamine moieties using a carbodiimide reaction. Flow cytometry and fluorescence microscopy revealed the interaction of these nanospheres with macrophages. Results: The nanocarriers were taken up by murine primary macrophages using clathrin-mediated endocytosis. Co-culture of macrophages with carbohydrate-functionalized nanospheres led to their activation and production of pro-inflammatory cytokines. One dose of amphotericin B-loaded on mannan-functionalized nanospheres resulted in an efficacy VL therapy. Conclusion: This approach provides a promising therapy for VL and a new therapeutic nanoplatform for obligate intracellular pathogens. |
URI: | https://repositorio-aberto.up.pt/handle/10216/118191 |
Source: | Nanomedicine, vol.10(3), p. 387-403 |
Related Information: | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/113151/PT info:eu-repo/grantAgreement/FCT/5876-PPCDTI/113151/PT info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F37880%2F2007/PT |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | openAccess |
Appears in Collections: | I3S - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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Barros2015.pdf | 911.63 kB | Adobe PDF | View/Open |
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