Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/114551
Author(s): Lima, AC
Jung, M
Rusch, J
Usmani, A
Lopes, AM
Conrad, DF
Title: A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
Publisher: MYJoVE Corporation
Issue Date: 2017
Abstract: Fluorescence-activated cell sorting (FACS) has been one of the methods of choice to isolate enriched populations of mammalian testicular germ cells. Currently, it allows the discrimination of up to 9 murine germ cell populations with high yield and purity
Subject: Animals
Flow Cytometry/methods
Germ Cells/cytology
Germ Cells/metabolism| | Male
Mice
Spermatogenesis/physiology
Spermatogonia/cytology
Testis/cytology
Testis/metabolism
URI: http://hdl.handle.net/10216/114551
Source: J Vis Exp. (125)
Related Information: info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F51695%2F2011/PT
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Embargo End Date: 2019-07-12
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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