Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/114551| Author(s): | Lima, AC Jung, M Rusch, J Usmani, A Lopes, AM Conrad, DF |
| Title: | A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry |
| Publisher: | MYJoVE Corporation |
| Issue Date: | 2017 |
| Abstract: | Fluorescence-activated cell sorting (FACS) has been one of the methods of choice to isolate enriched populations of mammalian testicular germ cells. Currently, it allows the discrimination of up to 9 murine germ cell populations with high yield and purity |
| Subject: | Animals Flow Cytometry/methods Germ Cells/cytology Germ Cells/metabolism| | Male Mice Spermatogenesis/physiology Spermatogonia/cytology Testis/cytology Testis/metabolism |
| DOI: | 10.3791/55913 |
| URI: | http://hdl.handle.net/10216/114551 |
| Source: | J Vis Exp. (125) |
| Related Information: | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F51695%2F2011/PT |
| Document Type: | Artigo em Revista Científica Internacional |
| Rights: | restrictedAccess |
| Embargo End Date: | 2019-07-12 |
| Appears in Collections: | I3S - Artigo em Revista Científica Internacional |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Lima_et_al_2017_JoVE.pdf | 1.39 MB | Adobe PDF |  View/Open | |
| Lima_et_al_2017_JoVE.mp4 Restricted Access | 27.11 MB | Unknown | View/Open |
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