A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Utilize este identificador para referenciar este registo: https://hdl.handle.net/10216/114551

Autor(es):	Lima, AC Jung, M Rusch, J Usmani, A Lopes, AM Conrad, DF
Título:	A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
Editor:	MYJoVE Corporation
Data de publicação:	2017
Resumo:	Fluorescence-activated cell sorting (FACS) has been one of the methods of choice to isolate enriched populations of mammalian testicular germ cells. Currently, it allows the discrimination of up to 9 murine germ cell populations with high yield and purity
Assunto:	Animals Flow Cytometry/methods Germ Cells/cytology Germ Cells/metabolism\| \| Male Mice Spermatogenesis/physiology Spermatogonia/cytology Testis/cytology Testis/metabolism
DOI:	10.3791/55913
URI:	http://hdl.handle.net/10216/114551
Fonte:	J Vis Exp. (125)
Informação Relacionada:	info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F51695%2F2011/PT
Tipo de Documento:	Artigo em Revista Científica Internacional
Condições de Acesso:	restrictedAccess
Data Fim de Embargo:	2019-07-12
Aparece nas coleções:	I3S - Artigo em Revista Científica Internacional

Ficheiros deste registo:

Ficheiro	Descrição	Tamanho	Formato
Lima_et_al_2017_JoVE.pdf		1.39 MB	Adobe PDF	Ver/Abrir
Lima_et_al_2017_JoVE.mp4 Restricted Access		27.11 MB	Unknown	Ver/Abrir