Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/114551
Author(s): | Lima, AC Jung, M Rusch, J Usmani, A Lopes, AM Conrad, DF |
Title: | A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry |
Publisher: | MYJoVE Corporation |
Issue Date: | 2017 |
Abstract: | Fluorescence-activated cell sorting (FACS) has been one of the methods of choice to isolate enriched populations of mammalian testicular germ cells. Currently, it allows the discrimination of up to 9 murine germ cell populations with high yield and purity |
Subject: | Animals Flow Cytometry/methods Germ Cells/cytology Germ Cells/metabolism| | Male Mice Spermatogenesis/physiology Spermatogonia/cytology Testis/cytology Testis/metabolism |
URI: | http://hdl.handle.net/10216/114551 |
Source: | J Vis Exp. (125) |
Related Information: | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F51695%2F2011/PT |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Embargo End Date: | 2019-07-12 |
Appears in Collections: | I3S - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Lima_et_al_2017_JoVE.pdf | 1.39 MB | Adobe PDF | View/Open | |
Lima_et_al_2017_JoVE.mp4 Restricted Access | 27.11 MB | Unknown | Request a copy |
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