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Author(s): Mesquita, FS
Brito, C
Cabanes, D
Sousa, S
Title: Control of cytoskeletal dynamics during cellular responses to pore forming toxins.
Publisher: Taylor & Francis
Issue Date: 2017
Abstract: Following damage by pore forming toxins (PFTs) host cells engage repair processes and display profound cytoskeletal remodeling and concomitant plasma membrane (PM) blebbing. We have recently demonstrated that host cells utilize similar mechanisms to controlcytoskeletal dynamics in response to PFTs and during cell migration. This involves assembly of cortical actomyosin bundles, reorganisation of the endoplasmic reticulum (ER) network, and the interaction between the ER chaperone Gp96 and the molecular motor Non-muscle Myosin Heavy Chain IIA (NMHCIIA). Consequently, Gp96 regulates actomyosin activity, PM blebbing and cell migration, and protects PM integrity against PFTs. In addition, we observed that PFTs increase association of Gp96 and ER vacuoles with the cell surface or within PM blebs loosely attached to the cell body. Similarly, gut epithelial cells damaged by PFTs in vivo were shown to release microvilli structures or directly purge cytoplasmic content. Cytoplasmic purging involves profound cytoskeletal remodeling and ER vacuolation, suggesting that our observations recapitulate recovery processes in vivo. Here, we discuss our findings in light of the current understanding of PM repair mechanisms and in vivo recovery responses to PFTs.
Subject: Actomyosin
Endoplasmic reticulum chaperone
Listeriolysin O
Plasma membrane repair
Pore-forming toxin
Source: Communicative & integrative biology, vol. 10(5-6): e1349582
Related Information: info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F94458%2F2013/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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