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dc.creatorMesquita, FS
dc.creatorBrito, C
dc.creatorCabanes, D
dc.creatorSousa, S
dc.description.abstractFollowing damage by pore forming toxins (PFTs) host cells engage repair processes and display profound cytoskeletal remodeling and concomitant plasma membrane (PM) blebbing. We have recently demonstrated that host cells utilize similar mechanisms to controlcytoskeletal dynamics in response to PFTs and during cell migration. This involves assembly of cortical actomyosin bundles, reorganisation of the endoplasmic reticulum (ER) network, and the interaction between the ER chaperone Gp96 and the molecular motor Non-muscle Myosin Heavy Chain IIA (NMHCIIA). Consequently, Gp96 regulates actomyosin activity, PM blebbing and cell migration, and protects PM integrity against PFTs. In addition, we observed that PFTs increase association of Gp96 and ER vacuoles with the cell surface or within PM blebs loosely attached to the cell body. Similarly, gut epithelial cells damaged by PFTs in vivo were shown to release microvilli structures or directly purge cytoplasmic content. Cytoplasmic purging involves profound cytoskeletal remodeling and ER vacuolation, suggesting that our observations recapitulate recovery processes in vivo. Here, we discuss our findings in light of the current understanding of PM repair mechanisms and in vivo recovery responses to PFTs.
dc.description.sponsorshipThis work was supported by national funds through FCT - Fundac¸ão para a Ciência e a Tecnologia / MEC - Ministério da Educac¸ão e Ciência and co-funded by FEDER-COMPETE 2020 and NORTE 2020 within projects POCI-01-0145-FEDER-007274, NORTE-01-0145-FEDER-000012, and Infect ERA/0001/2013 PROANTILIS. F. S. M. and C. B. were supported by FCT fellowships (SFRH/BPD/94458/2013, SFRH/ BD/112217/2015). S. S. received supported from FCT Investigator program (COMPETE, POPH, and FCT).
dc.publisherTaylor & Francis
dc.relation.ispartofCommunicative & integrative biology, vol. 10(5-6): e1349582
dc.subjectEndoplasmic reticulum chaperone
dc.subjectListeriolysin O
dc.subjectPlasma membrane repair
dc.subjectPore-forming toxin
dc.titleControl of cytoskeletal dynamics during cellular responses to pore forming toxins.
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoInstituto de Investigação e Inovação em Saúde
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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