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Author(s): A. Catarina Queiroga
Manuela E. Pintado
F. Xavier Malcata
Title: Wool-associated proteolytic bacteria, isolated from Portuguese Merino breed
Issue Date: 2013
Abstract: The main purpose of this study was to isolate and briefly characterize proteolytic bacteria from a poorly known habitat - raw wool. Fleece samples were accordingly collected from Merino raw wool - a Portuguese ewe breed, at three distinct areas of their body, from animals exhibiting no symptoms or signs of abnormalities; they were then subjected to enumeration and isolation of a total of 158 bacterial strains. Said isolates were screened for protease activity, using the spot technique, on Calcium Caseinate Agar containing 1% (w/v) skim milk. The 36 isolates displaying the highest protease activity underwent a more refined assessment of enzymatic performance - by examining their cell-free supernatant extracts, using casein as substrate. Two Bacillus isolates were eventually chosen owing to their highest proteolytic activities (24.6 and 15.9. U/mL), and identified using molecular biology tools.
Subject: Ciências da engenharia e tecnologias
Engineering and technology
Scientific areas: Ciências da engenharia e tecnologias
Engineering and technology
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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