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Author(s): Joana M. O. Santos
Mara Fernandes
Rita Araújo
Hugo Sousa
Joana Ribeiro
Margarida M. S. M. Bastos
Paula A. Oliveira
Diogo Carmo
Fátima Casaca
Sandra Silva
Ana L. Teixeira
Rui M. Gil da Costa
Rui Medeiros
Title: Dysregulated expression of microRNA-150 in human papillomavirus-induced lesions of K14-HPV16 transgenic mice
Issue Date: 2017
Abstract: Aims: High-risk human papillomavirus (HPV) infection is one of the major causes of infection-related cancersworldwide. MicroRNAs (miRNAs) are a family of non-coding RNAs (ncRNAs), whose dysregulated levels maycause an aberrant expression of genes involved in oncogenic pathways and consequently lead to cancer development. This is the case of the miRNA-150 (miR-150), whose expression in HPV-induced lesions remains unclearand the present work aims to clarify it. We employed K14-HPV16 mice, which express the early genes ofHPV16 in basal keratinocytes, leading to the development of hyperplastic and dysplastic skin lesions and squamous cell carcinomas, and are a representative model of HPV-induced cancers.Main methods: In order to evaluate the expression of miR-150 in HPV-induced lesions, we performed qPCR inwild-type mice (HPV−/−) and in skin lesions of K14-HPV16 transgenic mice (HPV+/−). Matched skin sampleswere analyzed histologically.Key findings: 24-26 weeks-old HPV+/− mice showed diffuse epidermal hyperplasia and focal dysplasia in a hyperplastic background (31.8% incidence), but 28-30 weeks-old HPV+/− mice presented higher incidence of dysplasia (100.0%). MiR-150 was upregulated in HPV+/− mice when compared with HPV−/− mice (p b 0.001). MiR150 was also overexpressed in diffuse dysplastic lesions when compared with hyperplastic lesions (p = 0.005).Significance: The present results suggest that miR-150 is overexpressed in HPV-induced lesions in this model andits expression seems to increase with lesion progression, along the process of multi-step carcinogenesis.
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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