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https://hdl.handle.net/10216/104673
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DC Field | Value | Language |
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dc.creator | Joana M. O. Santos | |
dc.creator | Mara Fernandes | |
dc.creator | Rita Araújo | |
dc.creator | Hugo Sousa | |
dc.creator | Joana Ribeiro | |
dc.creator | Margarida M. S. M. Bastos | |
dc.creator | Paula A. Oliveira | |
dc.creator | Diogo Carmo | |
dc.creator | Fátima Casaca | |
dc.creator | Sandra Silva | |
dc.creator | Ana L. Teixeira | |
dc.creator | Rui M. Gil da Costa | |
dc.creator | Rui Medeiros | |
dc.date.accessioned | 2023-08-24T23:05:28Z | - |
dc.date.available | 2023-08-24T23:05:28Z | - |
dc.date.issued | 2017 | |
dc.identifier.issn | 0024-3205 | |
dc.identifier.other | sigarra:195943 | |
dc.identifier.uri | https://hdl.handle.net/10216/104673 | - |
dc.description.abstract | Aims: High-risk human papillomavirus (HPV) infection is one of the major causes of infection-related cancers worldwide. MicroRNAs (miRNAs) are a family of non-coding RNAs (ncRNAs), whose dysregulated levels may cause an aberrant expression of genes involved in oncogenic pathways and consequently lead to cancer development. This is the case of the miRNA-150 (miR-150), whose expression in HPV-induced lesions remains unclear and the present work aims to clarify it. We employed K14-HPV16 mice, which express the early genes of HPV16 in basal keratinocytes, leading to the development of hyperplastic and dysplastic skin lesions and squamous cell carcinomas, and are a representative model of HPV-induced cancers. Main methods: In order to evaluate the expression of miR-150 in HPV-induced lesions, we performed qPCR in wild-type mice (HPV-/-) and in skin lesions of K14-HPV16 transgenic mice (HPV+/-). Matched skin samples were analyzed histologically. Key findings: 24-26 weeks-old HPV+/- mice showed diffuse epidermal hyperplasia and focal dysplasia in a hyperplastic background (31.8% incidence), but 28-30 weeks-old HPV+/- mice presented higher incidence of dysplasia (100.0%). MiR-150 was upregulated in HPV-/- mice when compared with HPV-/- mice (p < 0.001). MiR150 was also overexpressed in diffuse dysplastic lesions when compared with hyperplastic lesions (p = 0.005). Significance: The present results suggest that miR-150 is overexpressed in HPV-induced lesions in this model and its expression seems to increase with lesion progression, along the process of multi-step carcinogenesis. | |
dc.language.iso | eng | |
dc.relation | info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE | |
dc.rights | restrictedAccess | |
dc.title | Dysregulated expression of microRNA-150 in human papillomavirus-induced lesions of K14-HPV16 transgenic mice | |
dc.type | Artigo em Revista Científica Internacional | |
dc.contributor.uporto | Faculdade de Engenharia | |
dc.identifier.doi | 10.1016/j.lfs.2017.03.008 | |
dc.identifier.authenticus | P-00M-MXV | |
Appears in Collections: | FEUP - Artigo em Revista Científica Internacional |
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File | Description | Size | Format | |
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195943.pdf Restricted Access | Artigo original publicado | 738.37 kB | Adobe PDF | Request a copy from the Author(s) |
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