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https://hdl.handle.net/10216/104644
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DC Field | Value | Language |
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dc.creator | Laura Cerqueira | |
dc.creator | Ricardo M. Fernandes | |
dc.creator | Rui M. Ferreira | |
dc.creator | Mónica Oleastro | |
dc.creator | Fátima Carneiro | |
dc.creator | Catarina Brandão | |
dc.creator | Pedro Pimentel Nunes | |
dc.creator | Mário Dinis Ribeiro | |
dc.creator | Céu Figueiredo | |
dc.creator | Charles W. Keevil | |
dc.creator | Maria J. Vieira | |
dc.creator | Nuno F. Azevedo | |
dc.date.accessioned | 2022-09-16T08:28:46Z | - |
dc.date.available | 2022-09-16T08:28:46Z | - |
dc.date.issued | 2013 | |
dc.identifier.issn | 0095-1137 | |
dc.identifier.other | sigarra:104718 | |
dc.identifier.uri | https://hdl.handle.net/10216/104644 | - |
dc.description.abstract | Here, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determine H. pylori clarithromycin resistance. In the retrospective study (n = 30 patients), full agreement between PNA-FISH and PCR-sequencing was observed. Compared to the reference method (culture followed by Etest), the specificity and sensitivity of PNA-FISH were 90.9% (95% confidence interval [CI], 57.1% to 99.5%) and 84.2% (95% CI, 59.5% to 95.8%), respectively. In the prospective cohort (n = 93 patients), 21 cases were positive by culture. For the patients harboring clarithromycin-resistant H. pylori, the method showed sensitivity of 80.0% (95% CI, 29.9% to 98.9%) and specificity of 93.8% (95% CI, 67.7% to 99.7%). These values likely represent underestimations, as some of the discrepant results corresponded to patients infected by more than one strain. PNA-FISH appears to be a simple, quick, and accurate method for detecting H. pylori clarithromycin resistance in paraffin-embedded biopsy specimens. It is also the only one of the methods assessed here that allows direct and specific visualization of this microorganism within the biopsy specimens, a characteristic that allowed the observation that cells of different H. pylori strains can subsist in very close proximity in the stomach. | |
dc.language.iso | eng | |
dc.relation | info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projectos de Investigação Clínica/PIC/IC/82815/2007/Desenvolvimento e aplicação de mímicos de DNA para a rápida identificação de microrganismos patogénicos/DNA mimics | |
dc.rights | restrictedAccess | |
dc.subject | Ciências biológicas | |
dc.subject | Biological sciences | |
dc.title | Validation of a Fluorescence In Situ Hybridization Method Using Peptide Nucleic Acid Probes for Detection of Helicobacter pylori Clarithromycin Resistance in Gastric Biopsy Specimens | |
dc.type | Artigo em Revista Científica Internacional | |
dc.contributor.uporto | Faculdade de Engenharia | |
dc.contributor.uporto | Faculdade de Medicina | |
dc.identifier.doi | 10.1128/jcm.00302-13 | |
dc.identifier.authenticus | P-005-095 | |
dc.subject.fos | Ciências exactas e naturais::Ciências biológicas | |
dc.subject.fos | Natural sciences::Biological sciences | |
Appears in Collections: | FEUP - Artigo em Revista Científica Internacional FMUP - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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104718.pdf Restricted Access | Artigo original publicado | 645.62 kB | Adobe PDF | Request a copy from the Author(s) |
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